Li Duo, Qiao Zhenzhen, Yu Yanru, Tang Jinlu, He Xiaoxiao, Shi Hui, Ye Xiaosheng, Lei Yanli, Wang Kemin
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, Hunan 410082, China.
Chem Commun (Camb). 2018 Jan 25;54(9):1089-1092. doi: 10.1039/c7cc08228b.
A facile, general and turn-on nucleus imaging strategy was first developed based on in situ fluorescence activation of C-rich dark silver nanoclusters by G-rich telomeres. After a simple incubation without washing, nanoclusters could selectively stain the nucleus with intense red luminescence, which was confirmed using fixed/living cells and several cell lines.
基于富含G的端粒对富含C的暗银纳米团簇的原位荧光激活，首次开发了一种简便、通用且开启式的细胞核成像策略。经过简单孵育而无需洗涤后，纳米团簇能够用强烈的红色发光选择性地对细胞核进行染色，这在固定/活细胞和几种细胞系中得到了证实。
