DNA 银纳米簇的原位荧光激活作为一种无标记的细胞核成像通用策略。
In situ fluorescence activation of DNA-silver nanoclusters as a label-free and general strategy for cell nucleus imaging.
作者信息
Li Duo, Qiao Zhenzhen, Yu Yanru, Tang Jinlu, He Xiaoxiao, Shi Hui, Ye Xiaosheng, Lei Yanli, Wang Kemin
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, Hunan 410082, China.
出版信息
Chem Commun (Camb). 2018 Jan 25;54(9):1089-1092. doi: 10.1039/c7cc08228b.
A facile, general and turn-on nucleus imaging strategy was first developed based on in situ fluorescence activation of C-rich dark silver nanoclusters by G-rich telomeres. After a simple incubation without washing, nanoclusters could selectively stain the nucleus with intense red luminescence, which was confirmed using fixed/living cells and several cell lines.
基于富含G的端粒对富含C的暗银纳米团簇的原位荧光激活，首次开发了一种简便、通用且开启式的细胞核成像策略。经过简单孵育而无需洗涤后，纳米团簇能够用强烈的红色发光选择性地对细胞核进行染色，这在固定/活细胞和几种细胞系中得到了证实。
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