Yang Min, Chen Xu, Su Yuan, Liu Haiyan, Zhang Hongxing, Li Xiangyang, Xu Wentao
Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Faculty of Food Science and Engineering, Beijing University of Agriculture, Beijing, China.
Institute of Nutrition and Health, China Agricultural University, Beijing, China.
Front Chem. 2020 Nov 11;8:601621. doi: 10.3389/fchem.2020.601621. eCollection 2020.
Recently years have witnessed a surge in application of DNA-AgNCs in optics, catalysis, sensing, and biomedicine. DNA-templated silver nanoclusters (DNA-AgNCs), as emerging fluorophores, display superior optical performance since their size is close to the Fermi wavelength. DNA-AgNCs possess unique features, including high fluorescence quantum yields and stability, biocompatibility, facile synthesis, and low toxicity, which are requisite for fluorescent probes. The fluorescent emission of DNA-AgNCs can cover the violet to near-infrared (NIR) region by varying the DNA sequences, lengths, and structures or by modifying the environmental factors (such as buffer, pH, metal ions, macromolecular polymers, and small molecules). In view of the above excellent properties, we overview the DNA-AgNCs from the viewpoints of synthesis and fluorescence properties, and summarized its biological applications of fluorescence sensing and imaging.
近年来，DNA-AgNCs在光学、催化、传感和生物医学领域的应用激增。DNA模板化银纳米簇（DNA-AgNCs）作为新兴的荧光团，由于其尺寸接近费米波长，表现出优异的光学性能。DNA-AgNCs具有独特的特性，包括高荧光量子产率和稳定性、生物相容性、易于合成和低毒性，这些都是荧光探针所必需的。通过改变DNA序列、长度和结构，或通过改变环境因素（如缓冲液、pH值、金属离子、大分子聚合物和小分子），DNA-AgNCs的荧光发射可以覆盖从紫色到近红外（NIR）区域。鉴于上述优异性能，我们从合成和荧光性质的角度对DNA-AgNCs进行了综述，并总结了其在荧光传感和成像方面的生物学应用。
