Department of Urology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, P.R. China.
Oncol Rep. 2018 Mar;39(3):1454-1460. doi: 10.3892/or.2018.6209. Epub 2018 Jan 11.
Prostate cancer (PCa) is the most commonly diagnosed male malignancy and the second leading cause of male cancer-related deaths. miR-141 has been demonstrated to be inversely correlated with tumorigenicity. In the present study, we investigated the effect of miR-141 and runt-related transcription factor 1 (RUNX1) on PCa cells. We determined that miR-141 was expressed at a low level and RUNX1 was expressed at a high level in PCa tissues in comparison to that in adjacent normal tissues. Upregulation of miR-141 significantly inhibited cell growth, migration and invasion, and promoted cell apoptosis in PCa cells. Furthermore, miR-141 overexpression suppressed the expression of MMP-2 and MMP-9, and increased the expression of FOXO1 and p21. However, overexpression of RUNX1 could antagonize the effects of miR-141 on PCa cells. Our findings demonstrated that miR-141 could suppress cell growth, migration and invasion and induce cell apoptosis by targeting RUNX1 in PCa cells. Thus, miR-141/RUNX1 play critical roles in the progression of PCa and may be promising targets for the diagnosis and treatment of PCa.
前列腺癌（PCa）是最常见的男性恶性肿瘤，也是男性癌症相关死亡的第二大主要原因。miR-141 已被证明与致瘤性呈负相关。在本研究中，我们研究了 miR-141 和 runt 相关转录因子 1（RUNX1）对 PCa 细胞的影响。与相邻正常组织相比，我们发现 miR-141 在 PCa 组织中表达水平较低，而 RUNX1 表达水平较高。miR-141 的上调显著抑制了 PCa 细胞的生长、迁移和侵袭，并促进了细胞凋亡。此外，miR-141 的过表达抑制了 MMP-2 和 MMP-9 的表达，并增加了 FOXO1 和 p21 的表达。然而，RUNX1 的过表达可以拮抗 miR-141 对 PCa 细胞的作用。我们的研究结果表明，miR-141 可以通过靶向 RUNX1 抑制 PCa 细胞的生长、迁移和侵袭，并诱导细胞凋亡。因此，miR-141/RUNX1 在 PCa 的进展中起关键作用，可能成为 PCa 诊断和治疗的有希望的靶点。
