Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Department of Pathology and Physiology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Mol Med Rep. 2018 Mar;17(3):4369-4375. doi: 10.3892/mmr.2018.8422. Epub 2018 Jan 11.
The present study aimed to explore the therapeutic effects of cyclosporin A (CsA) on spinal cord injury (SCI) in rats with hyperglycemia and to identify a novel potential method to treat SCI in the presence of hyperglycemia. Female Sprague‑Dawley (SD) rats were randomly allocated into four groups: Sham, SCI, SCI+hyperglycemia and SCI+hyperglycemia+CsA groups. Streptozotocin‑induced hyperglycemic SD rats and a weight‑drop contusion SCI model were established. The Basso, Beattie, Bresnahan scale and inclined plane test were used to evaluate the neurological function of the rats. Flow cytometric assay was performed to detect the apoptotic rates of cells in the spinal cord. ELISA and western blot analysis were performed to determine the levels of interleukin (IL)‑10, tumor necrosis factor (TNF)‑α, cyclophilin‑D (Cyp‑D) and apoptosis‑inducing factor (AIF). The results demonstrated that CsA significantly improved the neurological function of the SCI rats with hyperglycemia. CsA markedly reduced the number of apoptotic cells exaggerated by hyperglycemia in the spinal cord of the SCI rats. CsA significantly decreased the expression levels of IL‑10, TNF‑α, Cyp‑D and AIF in the spinal cord of the SCI rats. Overall, the present study revealed a significant role of CsA in the treatment of SCI in the presence of hyperglycemia by inhibiting the apoptosis of spinal cord cells.
本研究旨在探讨环孢素 A（CsA）对高血糖大鼠脊髓损伤（SCI）的治疗作用，并确定一种治疗高血糖合并 SCI 的新方法。将雌性 Sprague-Dawley（SD）大鼠随机分为四组：假手术组、SCI 组、高血糖合并 SCI 组和高血糖合并 SCI+CsA 组。建立链脲佐菌素诱导的高血糖 SD 大鼠和重物坠落挫伤 SCI 模型。Basso、Beattie、Bresnahan 量表和斜面试验用于评估大鼠的神经功能。流式细胞术检测脊髓细胞的凋亡率。ELISA 和 Western blot 分析用于检测白细胞介素（IL）-10、肿瘤坏死因子（TNF）-α、亲环蛋白 D（Cyp-D）和凋亡诱导因子（AIF）的水平。结果表明，CsA 可显著改善高血糖合并 SCI 大鼠的神经功能。CsA 显著减少了高血糖加重的 SCI 大鼠脊髓中凋亡细胞的数量。CsA 显著降低了 SCI 大鼠脊髓中 IL-10、TNF-α、Cyp-D 和 AIF 的表达水平。综上所述，本研究揭示了 CsA 通过抑制脊髓细胞凋亡在治疗高血糖合并 SCI 中具有重要作用。
