Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea.
Mol Med Rep. 2018 Mar;17(3):4399-4405. doi: 10.3892/mmr.2018.8401. Epub 2018 Jan 9.
4-Hydroxy-3-methoxybenzaldehyde (vanillin), contained in a number of species of plant, has been reported to display beneficial effects against brain injuries. In the present study, the impact of vanillin on scopolamine‑induced alterations in cognition and the expression of DNA binding protein inhibitor ID‑1 (ID1), one of the inhibitors of DNA binding/differentiation proteins that regulate gene transcription, in the mouse hippocampus. Mice were treated with 1 mg/kg scopolamine with or without 40 mg/kg vanillin once daily for 4 weeks. Scopolamine‑induced cognitive impairment was observed from 1 week and was deemed to be severe 4 weeks following the administration of scopolamine. However, treatment with vanillin in scopolamine‑treated mice markedly attenuated cognitive impairment 4 weeks following treatment with scopolamine. ID1‑immunoreactive cells were revealed in the hippocampus of vehicle‑treated mice, and were hardly detected 4 weeks following treatment with scopolamine. However, treatment with vanillin in scopolamine‑treated mice markedly restored ID1‑immunoreactive cells and expression 4 weeks subsequent to treatment. The results of the present study suggested that vanillin may be beneficial for cognitive impairment, by preventing the reduction of ID1 expression which may be associated with cognitive impairment.
4-羟基-3-甲氧基苯甲醛（香草醛）存在于多种植物中，据报道具有对抗脑损伤的有益作用。在本研究中，研究了香草醛对东莨菪碱诱导的认知改变以及 DNA 结合蛋白抑制剂 ID-1（ID1）表达的影响，ID1 是调节基因转录的 DNA 结合/分化蛋白抑制剂之一。小鼠每日用 1mg/kg 东莨菪碱加或不加 40mg/kg 香草醛处理 4 周。从第 1 周开始观察到东莨菪碱诱导的认知障碍，并且在给予东莨菪碱后 4 周时被认为是严重的。然而，用香草醛治疗在东莨菪碱处理的小鼠中显著减轻了 4 周后用东莨菪碱处理后的认知障碍。在载体处理的小鼠的海马体中显示出 ID1 免疫反应性细胞，并且在用东莨菪碱处理 4 周后几乎未检测到。然而，用香草醛治疗在东莨菪碱处理的小鼠中显著恢复了 ID1 免疫反应性细胞和表达 4 周后。本研究的结果表明，香草醛可能通过防止 ID1 表达的减少而对认知障碍有益，ID1 表达的减少可能与认知障碍有关。
