Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150086, China; The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Harbin, Heilongjiang Province, 150086, China.
The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Harbin, Heilongjiang Province, 150086, China.
Biomed Pharmacother. 2018 Mar;99:121-127. doi: 10.1016/j.biopha.2018.01.061. Epub 2018 Jan 10.
Cholangiocarcinoma (CCA) is one of the most aggressive malignancies in humans. Emerging evidence has indicated that abnormally expressed long non-coding RNAs (lncRNAs) could conduce to tumorigenesis and progression. Specifically, colon cancer-associated transcript 2 (CCAT2) has been reported to be overexpressed in several carcinomas. However, its clinical significance and functional roles in CCA is still unknown. qRT-PCR experiments were conducted to assess the CCAT2 expression in CCA tissue samples and cell lines. In addition, the link between CCAT2 expression and clinicopathological characteristics was analyzed. The potential effects of CCAT2 in CCA cells was evaluated in vitro including cell proliferation, colony-forming ability, apoptosis, migration, invasion and epithelial-to-mesenchymal transition (EMT). As a result, CCAT2 was aberrantly overexpressed in CCA tissue samples and cells, and this upregulation was correlated with tumor size, lymph node invasion, TNM stage and postoperative recurrence in CCA patients. Overexpression of CCAT2 could serve as an independent prognostic indicator for CCA. Additionally, overexpression of CCAT2 was a dismal prognostic indicator for patients with CCA. Furthermore, CCAT2 silencing caused tumor suppressive effects via reducing cell proliferation, migration and invasion, inducing cell apoptosis and reversing the EMT process in HuCCT1 and CCLP1 cells. Collectively, our data illustrated that lncRNA CCAT2 played an oncogenic role in CCA and may offer a potential therapeutic target for treating this fatal disease.
胆管癌（CCA）是人类最具侵袭性的恶性肿瘤之一。新出现的证据表明，异常表达的长非编码 RNA（lncRNA）可能导致肿瘤发生和进展。具体来说，结肠癌相关转录物 2（CCAT2）已在几种癌中被报道过表达。然而，其在 CCA 中的临床意义和功能作用仍不清楚。qRT-PCR 实验用于评估 CCA 组织样本和细胞系中的 CCAT2 表达。此外，还分析了 CCAT2 表达与临床病理特征之间的联系。在体外评估了 CCAT2 在 CCA 细胞中的潜在作用，包括细胞增殖、集落形成能力、凋亡、迁移、侵袭和上皮间质转化（EMT）。结果表明，CCAT2 在 CCA 组织样本和细胞中异常过表达，这种上调与 CCA 患者的肿瘤大小、淋巴结浸润、TNM 分期和术后复发有关。CCAT2 的过表达可以作为 CCA 的独立预后指标。此外，CCAT2 的过表达是 CCA 患者预后不良的指标。此外，CCAT2 沉默通过减少 HuCCT1 和 CCLP1 细胞的增殖、迁移和侵袭，诱导细胞凋亡和逆转 EMT 过程，发挥肿瘤抑制作用。总之，我们的数据表明，lncRNA CCAT2 在 CCA 中发挥致癌作用，可能为治疗这种致命疾病提供潜在的治疗靶点。
