Zhu Xiaolin, Zhang Kexin, Lu Nan, Yu Yangyang, Yuan Xing, Guan Jiunian
School of Environment, Northeast Normal University, Changchun, 130117, PR China.
School of Environment, Northeast Normal University, Changchun, 130117, PR China.
Chemosphere. 2018 Apr;196:514-521. doi: 10.1016/j.chemosphere.2018.01.006. Epub 2018 Jan 4.
o-Chlorophenol (OCP) is a priority pollutant that poses serious health threats to the public. The following study designs a simple electrochemical system to monitor the concentration and toxicity of OCP. This system was primarily characterized by the integration of both physicochemical and biological monitoring procedures that had a synergistic effect between the functionalized carbon nanotubes and rhodamine B. This resulted in excellent electrocatalytic activities toward OCP and cellular purine bases. The peak current of OCP was linear with concentrations ranging from 0.05-125.0 μM and the detection limit was 0.028 μM under optimal testing conditions. There was an enhanced voltammetric signal detected that was caused by the guanine/xanthine of human hepatoma (HepG2) cells. The cytotoxicity of OCP to HepG2 cells was assessed using the proposed system. The obtained IC value was 512.86 μM. This study provided a fast, label-free, and low-cost platform for the comprehensive assessment of OCP. This is highly beneficial for simplifying the environmental monitoring process.
邻氯苯酚（OCP）是一种对公众健康构成严重威胁的优先污染物。以下研究设计了一种简单的电化学系统来监测OCP的浓度和毒性。该系统的主要特点是整合了物理化学和生物监测程序，功能化碳纳米管与罗丹明B之间具有协同效应。这导致了对OCP和细胞嘌呤碱基具有出色的电催化活性。在最佳测试条件下，OCP的峰值电流与浓度在0.05 - 125.0 μM范围内呈线性关系，检测限为0.028 μM。检测到由人肝癌（HepG2）细胞的鸟嘌呤/黄嘌呤引起的伏安信号增强。使用所提出的系统评估了OCP对HepG2细胞的细胞毒性。获得的IC值为512.86 μM。本研究为OCP的综合评估提供了一个快速、无标记且低成本的平台。这对于简化环境监测过程非常有益。
