School of Environment, Northeast Normal University, Changchun, 130117, People's Republic of China.
Environ Monit Assess. 2019 Jul 27;191(8):516. doi: 10.1007/s10661-019-7660-4.
In this study, the concentrations of nine typical antibiotics, including sulfadiazine (SD), sulfamerazine (SMR), sulfamethazine (SM2), sulfamethoxazole (SMZ), ofloxacin (OFX), ciprofloxacin (CIP), trimethoprim (TMP), oxytetracycline (OTC), and tetracycline hydrochloride (TC), were detected in the Yitong River by solid-phase extraction high-performance liquid chromatography. The concentrations of the antibiotics were analyzed. Additionally, an improved immobilized substrate enzyme substrate method (DST-enzyme substrate method) was developed and used to evaluate the antibiotic resistance of coliform bacteria to OFX, CIP, enrofloxacin (ENR), TC, sulfisoxazole (SOX), and TMP in the Yitong River. The results showed that the concentrations of the nine antibiotics ranged from nd (not detected) to 1.361 μg/L. The detection rate and concentration of OFX were the highest, followed by CIP, and the detection rate and concentration of SM2 and OTC were the lowest. The detection rate and concentrations of antibiotics were higher in August and November than those in May. The antibiotics were mainly distributed in the livestock sewage discharge and suburban domestic sewage discharge areas. Moreover, the drug resistance of total coliform bacteria to fluoroquinolones, sulfonamides, tetracyclines, and TMP varied with season.
本研究采用固相萃取-高效液相色谱法检测了伊通河中 9 种典型抗生素（磺胺嘧啶（SD）、磺胺甲噁唑（SMR）、磺胺二甲嘧啶（SM2）、磺胺甲噁唑（SMZ）、氧氟沙星（OFX）、环丙沙星（CIP）、甲氧苄啶（TMP）、土霉素（OTC）和盐酸四环素（TC））的浓度。分析了抗生素的浓度。此外，还开发并应用了一种改良的固定化基质酶底物法（DST-酶底物法）来评估伊通河中大肠菌群对抗生素 OFX、CIP、恩诺沙星（ENR）、TC、磺胺二甲嘧啶（SOX）和 TMP 的耐药性。结果表明，9 种抗生素的浓度范围为未检出（nd）至 1.361μg/L。OFX 的检出率和浓度最高，其次是 CIP，SM2 和 OTC 的检出率和浓度最低。8 月和 11 月的抗生素检出率和浓度高于 5 月。抗生素主要分布在牲畜污水排放和郊区生活污水排放区。此外，总大肠菌群对抗菌药物（氟喹诺酮类、磺胺类、四环素类和 TMP）的耐药性随季节而变化。
