Weselcouch E O, Humphrey W R, Aiken J W
Am J Physiol. 1985 Nov;249(5 Pt 2):R595-602. doi: 10.1152/ajpregu.1985.249.5.R595.
The effect of passage through the pulmonary and renal circulation on the activity of extracted and synthetic atrial natriuretic factors was determined by monitoring the amount of the vascular relaxing activity surviving passage through the organ. When crude atrial extract was infused through isolated perfused lungs of the guinea pig, approximately 75% of its activity survived. The activity of a 23-amino acid synthetic atrial peptide was decreased 21% on passage through these lungs, but this loss of activity was not significantly different from the crude extract. Analogous experiments in vivo showed that passage through the pulmonary circulation of the dog did not alter the activity of either the crude atrial extract or of synthetic atrial peptide. In contrast to the lack of effect of the pulmonary circulation, approximately 80% of the activity of crude atrial extract and synthetic atrial peptide was removed by the isolated perfused kidney of the rabbit, and in vivo the dog kidney removed approximately 80% of the activity of both these atrial substances. By surviving transit through the pulmonary circulation, these materials exhibit a necessary property of a circulating hormone stored in the right atrium, and the fact that the renal circulation extracts most of these materials is consistent with the kidney being a target organ of this putative hormone.
通过监测经器官循环后仍保留的血管舒张活性量，来确定经肺循环和肾循环对提取及合成的心房利钠因子活性的影响。当将粗制心房提取物注入豚鼠离体灌注肺时，其约75%的活性得以保留。一种23个氨基酸的合成心房肽经这些肺循环后活性降低了21%，但这种活性损失与粗制提取物相比无显著差异。体内的类似实验表明，经犬的肺循环后，粗制心房提取物或合成心房肽的活性均未改变。与肺循环无影响相反，兔的离体灌注肾可去除约80%的粗制心房提取物和合成心房肽的活性，在体内犬肾可去除这两种心房物质约80%的活性。通过在肺循环中存活，这些物质展现出作为储存在右心房的循环激素的必要特性，并且肾循环能提取大部分这些物质这一事实与肾脏是这种假定激素的靶器官相一致。
