Qin Chuanjie, Gong Quan, Wen Zhengyong, Yuan Dengyue
College of Life Science, Neijiang Normal University, Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang 641000, PR China.
Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 611731, PR China.
Dev Comp Immunol. 2018 May;82:66-71. doi: 10.1016/j.dci.2018.01.004. Epub 2018 Jan 9.
Complement factor I (CFI) is a novel regulatory serine protease that plays an important role in resistance to pathogen infection. In this study, the CFI gene of Pelteobagrus vachellii (Pv-CFI) was sequenced and characterized. The full-length cDNA of 2320 bp includes a 155 bp 5'-untranslated region (UTR), a 164 bp 3'-UTR, and a 2001 bp open reading frame (ORF) encoding a 667 amino acids. Multiple sequence alignment revealed five highly conserved domains with a typical modular architecture and identical active sites in vertebrates, indicating a conserved function. Pv-CFI mRNA was constitutively expressed in all examined tissues and most abundant in liver. During infection with Aeromonas hydrophila, Pv-CFI mRNA expression was significantly up-regulated in liver at 3-24 h, spleen at 3-48 h and head kidney at 3-48 h. The results suggest Pv-CFI plays an important role in resistance to pathogenic bacteria in P. vachellii.
补体因子I（CFI）是一种新型的调节性丝氨酸蛋白酶，在抵抗病原体感染中发挥重要作用。在本研究中，对瓦氏黄颡鱼（Pelteobagrus vachellii）的CFI基因（Pv-CFI）进行了测序和特征分析。2320 bp的全长cDNA包括一个155 bp的5'非翻译区（UTR）、一个164 bp的3'UTR和一个2001 bp的开放阅读框（ORF），编码667个氨基酸。多序列比对揭示了五个高度保守的结构域，具有典型的模块化结构和脊椎动物中相同的活性位点，表明其功能保守。Pv-CFI mRNA在所有检测组织中组成性表达，在肝脏中含量最高。在感染嗜水气单胞菌期间，Pv-CFI mRNA表达在肝脏中3-24小时、脾脏中3-48小时和头肾中3-48小时显著上调。结果表明Pv-CFI在瓦氏黄颡鱼抵抗病原菌方面发挥重要作用。
