Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, 641000, PR China; College of Life Science, Neijiang Normal University, Neijiang, 641000, PR China.
Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, 641000, PR China; College of Life Science, Neijiang Normal University, Neijiang, 641000, PR China.
Fish Shellfish Immunol. 2020 Apr;99:572-577. doi: 10.1016/j.fsi.2020.02.048. Epub 2020 Feb 26.
The pleiotropic transcription factor nuclear factor-kappa B (NF-κB) has important functions in viral resistance. In the present study, we isolated a p65 subunit of NF-κB cDNA from Pelteobagrus vachellii (termed Pvp65) and characterized it. The full-length p65 cDNA comprised 3651 bp, including a 148-bp 5'-untranslated region (UTR), a 106-bp 3'-UTR, and an open reading frame encoding a 1067-amino acid putative protein. The protein sequence comprised a DNA binding motif, a Rel-homology domain, a Rel protein signature, a putative transcription activation domain, a nuclear localization signal, and a transcription initiation factor IIA domain. The expression of Pvp65 displayed a daily rhythm, with an acrophase at approximately at 15:32 h in the liver, 11:34 h in the spleen, and 16:45 h in the head kidney. In addition, infection with Aeromonas hydrophila caused Pvp65 expression to increase significantly (P < 0.05), and peaking at 12 h post infection in the spleen, at 24 in the head kidney, and at 12 h in the liver. Thus, NF-κB expression might be under light/dark cycle control in P. vachellii, and may be involved in the immune response to A. hydrophila.
多效转录因子核因子-κB（NF-κB）在抗病毒中具有重要作用。本研究从黄颡鱼（Pelteobagrus vachellii）中分离出 NF-κB 的 p65 亚基 cDNA（命名为 Pvp65），并对其进行了鉴定。全长 p65 cDNA 包含 3651 bp，包括 148 bp 的 5'-非翻译区（UTR）、106 bp 的 3'-UTR 和一个开放阅读框，编码 1067 个氨基酸的假定蛋白。该蛋白序列包含一个 DNA 结合基序、一个 Rel 同源结构域、一个 Rel 蛋白签名、一个假定的转录激活结构域、一个核定位信号和一个转录起始因子 IIA 结构域。Pvp65 的表达呈昼夜节律，在肝脏中的峰值出现在约 15:32 h，在脾脏中的峰值出现在 11:34 h，在头肾中的峰值出现在 16:45 h。此外，感染嗜水气单胞菌后，Pvp65 的表达显著增加（P<0.05），在脾脏中 12 h 时达到峰值，在头肾中 24 h 时达到峰值，在肝脏中 12 h 时达到峰值。因此，NF-κB 的表达可能受到黄颡鱼光/暗周期的调控，并可能参与对嗜水气单胞菌的免疫反应。
