CBQF, Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 172, 4200-374 Porto, Portugal; INEB, Instituto Nacional de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen 208, 4200-393 Porto, Portugal.
CBQF, Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 172, 4200-374 Porto, Portugal; CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra 1317, 4585-116 Gandra-PRD, Portugal; INEB, Instituto Nacional de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen 208, 4200-393 Porto, Portugal.
Peptides. 2018 Mar;101:112-123. doi: 10.1016/j.peptides.2018.01.002. Epub 2018 Jan 9.
Bioactive proteins and peptides have been used with either prophylactic or therapeutic purposes, presenting inherent advantages as high specificity and biocompatibility. Nanocarriers play an important role in the stabilization of proteins and peptides, offering enhanced buccal permeation and protection while crossing the gastrointestinal tract. Moreover, preparation of nanoparticles as oral delivery systems for proteins/peptides may include tailored formulation along with functionalization aiming bioavailability enhancement of carried proteins or peptides. Oral delivery systems, namely buccal delivery systems, represent an interesting alternative route to parenteric delivery systems to carry proteins and peptides, resulting in higher comfort of administration and, therefore, compliance to treatment. This paper outlines an extensive overview of the existing publications on proteins/peptides oral nanocarriers delivery systems, with special focus on buccal route. Manufacturing aspects of most commonly used nanoparticles for oral delivery (e.g. polymeric nanoparticles using synthetic or natural polymers and lipid nanoparticles) advantages and limitations and potential applications of nanoparticles as proteins/peptides delivery systems will also be thoroughly addressed.
生物活性蛋白和肽已被用于预防或治疗目的，具有高特异性和生物相容性的固有优势。纳米载体在蛋白质和肽的稳定化中发挥着重要作用，提供了增强的口腔渗透和保护，同时穿过胃肠道。此外，制备纳米颗粒作为蛋白质/肽的口服递药系统可包括定制配方以及功能化，旨在提高所携带的蛋白质或肽的生物利用度。口服递药系统，即口腔递药系统，代表了一种与肠外递药系统相比，具有更高的舒适性和因此更高的治疗顺应性的载蛋白和肽的替代途径。本文概述了关于蛋白质/肽口服纳米载体递药系统的现有文献的广泛综述，特别关注口腔途径。还将详细讨论最常用于口服递药的纳米颗粒（例如使用合成或天然聚合物的聚合物纳米颗粒和脂质纳米颗粒）的制造方面、优势和局限性以及纳米颗粒作为蛋白质/肽递药系统的潜在应用。
