Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran.
Department of Veterinary Science, Rasht Branch, Islamic Azad University, Rasht, Iran.
Environ Sci Pollut Res Int. 2018 Mar;25(9):8836-8842. doi: 10.1007/s11356-018-1209-x. Epub 2018 Jan 12.
The aim of the present study was to evaluate the effect rosemary (Rosmarinus officinalis L.) powder (RP) and vitamin E (VE) at different levels on humoral immunity of broilers during a 42-day production cycle. A total of 270 1-day-old male chicks were assigned to nine groups with three replicates of ten birds each, and diets were supplemented with 0, 0.5, or 1.0% RP and 0, 100, or 200 mg/kg VE, respectively. Commercial-inactivated vaccines against avian influenza (AI) and Newcastle disease (ND) viruses, and living infectious bronchitis virus (IBV) vaccine were administered by spray method. Sheep red blood cells (SRBC) were administered subcutaneously. Blood samples were collected from birds 1 week after each vaccination to determine antibody titers. At the 42nd day, blood samples were also assessed for globulin level, and lymphoid tissues (thymus, spleen, and bursa) were weighed. Neither antibody titers against viruses nor lymphoid tissues weight were affected by RP and/or VE (P > 0.05) treatments. However, broilers supplemented with 0 mg/kg of VE had lower antibody titers against SRBC than those fed 100 mg/kg of VE (P < 0.05) at the 24th day. A significant RP × VE interaction effect (P < 0.05) on plasma globulin level was observed. The findings of our study suggest that dietary RP and VE additives can interact and modulate the humoral immunity of broilers, but not sufficiently to improve antibody titers against specific virus during a 42-day production cycle.
本研究旨在评估迷迭香（Rosmarinus officinalis L.）粉（RP）和维生素 E（VE）在不同水平下对肉鸡 42 天生产周期体液免疫的影响。将 270 只 1 日龄雄性小鸡分为 9 组，每组 10 只，每组饲料分别补充 0、0.5 或 1.0%的 RP 和 0、100 或 200mg/kg 的 VE。采用喷雾法接种鸡流感（AI）和新城疫（ND）病毒的商业灭活疫苗和传染性支气管炎病毒（IBV）活疫苗。绵羊红细胞（SRBC）皮下注射。在每次接种后 1 周从鸡中采集血液样本以确定抗体滴度。在第 42 天，还评估了血液球蛋白水平，并称重淋巴组织（胸腺、脾脏和法氏囊）。RP 和/或 VE（P>0.05）处理均未影响病毒抗体滴度或淋巴组织重量。然而，与补充 100mg/kg VE 的鸡相比，补充 0mg/kg VE 的鸡在第 24 天对 SRBC 的抗体滴度较低（P<0.05）。观察到血浆球蛋白水平存在显著的 RP×VE 互作效应（P<0.05）。本研究的结果表明，日粮 RP 和 VE 添加剂可以相互作用并调节肉鸡的体液免疫，但不足以在 42 天的生产周期内提高针对特定病毒的抗体滴度。
