美法仑体外对刀豆蛋白A诱导小鼠抑制性T细胞的影响。
Effect of melphalan in vitro on induction of murine suppressor T cells by ConA.
作者信息
Ophir R, Ben-Efraim S
出版信息
Cancer Immunol Immunother. 1985;20(3):209-13. doi: 10.1007/BF00205578.
The effect of treatment with melphalan in vitro on the activity of spleen cells from BALB/c mice was investigated. Incubation of spleen cells with 1.5-5 micrograms melphalan/1 X 10(7) inhibited subsequent mitogenic stimulation by ConA or PHA and the allogeneic response of BALB/c spleen cells against C57B1 target spleen cells. Incubation of spleen cells with ConA led to induction of suppressor T cells which when added to fresh cultures inhibited the allogeneic response. Preincubation of spleen cells with melphalan even at low concentrations (0.15-0.5 micrograms 1 X 10(7) cells) which do not directly affect mitogenic stimulation or allogeneic response partially inhibited the generation of suppressor T cells by ConA. Treatment with melphalan had no effect on already induced suppressor T cells as shown by incubation of spleen cells with melphalan (0.15-5 micrograms/1 X 10(7) cells) after incubation with ConA. Addition of cells treated with melphalan alone (without ConA) to fresh cultures led to an increase in the allogeneic response.
研究了美法仑体外处理对BALB/c小鼠脾细胞活性的影响。用1.5 - 5微克美法仑/1×10⁷脾细胞孵育，会抑制随后由刀豆蛋白A（ConA）或植物血凝素（PHA）介导的有丝分裂刺激以及BALB/c脾细胞对C57B1靶脾细胞的同种异体反应。脾细胞与ConA孵育会诱导抑制性T细胞的产生，将这些抑制性T细胞加入新鲜培养物中会抑制同种异体反应。脾细胞即使在不直接影响有丝分裂刺激或同种异体反应的低浓度（0.15 - 0.5微克/1×10⁷细胞）美法仑预孵育下，也会部分抑制ConA诱导的抑制性T细胞的产生。如在ConA孵育后用美法仑（0.15 - 5微克/1×10⁷细胞）孵育脾细胞所示，美法仑处理对已诱导的抑制性T细胞没有影响。将仅用美法仑处理（无ConA）的细胞加入新鲜培养物中会导致同种异体反应增强。
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