Benstead K, Moore J V
Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
Br J Cancer. 1988 May;57(5):451-4. doi: 10.1038/bjc.1988.105.
The clearance of an intradermally-injected solution of 133Xenon in 0.9% saline has been used to study the impairment and recovery of blood flow in mouse tail for 5 days following photodynamic therapy (PDT) with 2mg TPPS i.v. per mouse and a range of doses of white light. Impairment of blood flow was observed within 10 min of light exposure. Blood flow increased between day 1 and day 5 at light doses less than 151J cm-2 and had returned to control levels by day 5 at light doses less than 129J cm-2. In mice treated with a light dose that caused a 50% incidence of necrosis, there was no significant difference in the initial xenon clearance half-time (measured at 10 min and 1 day after PDT) between those mice which developed tail necrosis and those which healed. However, the latter showed significantly greater improvement in vascular function on days 2, 3 and 4. This suggests that the timing and extent of recovery of blood flow determined the risk of necrosis in individual mice.
已使用皮内注射含133氙的0.9%盐水溶液的清除率，来研究在每只小鼠静脉注射2mg四磺基苯卟啉（TPPS）并给予一系列白光剂量进行光动力疗法（PDT）后5天内小鼠尾部血流的损伤和恢复情况。在光照后10分钟内观察到血流受损。在小于151J/cm²的光剂量下，第1天到第5天血流增加，在小于129J/cm²的光剂量下，到第5天血流已恢复到对照水平。在用导致50%坏死发生率的光剂量治疗的小鼠中，发生尾部坏死的小鼠和愈合的小鼠之间，初始氙清除半衰期（在PDT后10分钟和1天测量）没有显著差异。然而，后者在第2、3和4天显示出血管功能有显著更大的改善。这表明血流恢复的时间和程度决定了个体小鼠坏死的风险。
