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Zhonghua Zhong Liu Za Zhi. 1986 Jan;8(1):32-4.
The mutagenic effect of HpD on cell SCE and the reactions of cell SCE to different sources of light combined with HpD were studied using V79 cells. There were 6 doses of HpD: 1 microgram/ml, 3 micrograms/ml, 5 micrograms/ml, 10 micrograms/ml, 50 micrograms/ml and 100 micrograms/ml. The dose of 5 micrograms/ml is equal to the maximum dose of HpD used in the clinic (HpD per milliliter of patient's blood). Our experiments demonstrated that when the cells were cultured in the dark and HpD was added to the medium no more than 5 micrograms/ml, the SCE frequencies were not increased. The cells were irradiated with different sources of light without HpD, both the fluorescence and ultraviolet light could promote SCE but the light of daylight lamp and red light did not increase it. But when HpD was added into culture medium at the dose of less than 5 micrograms/ml, every light could increase the cell SCE intensively except the daylight lamp light. The red light was more notable than the others by relation analysis.
利用V79细胞研究了血卟啉衍生物（HpD）对细胞姐妹染色单体交换（SCE）的诱变作用以及细胞SCE对不同光源与HpD联合作用的反应。HpD有6个剂量：1微克/毫升、3微克/毫升、5微克/毫升、10微克/毫升、50微克/毫升和100微克/毫升。5微克/毫升的剂量相当于临床上使用的HpD最大剂量（每毫升患者血液中的HpD）。我们的实验表明，当细胞在黑暗中培养且向培养基中添加的HpD不超过5微克/毫升时，SCE频率不会增加。在无HpD的情况下用不同光源照射细胞，荧光和紫外光均可促进SCE，但日光灯和红光照射不会使其增加。但是当以小于5微克/毫升的剂量将HpD添加到培养基中时，除日光灯外，每种光均可强烈增加细胞SCE。通过相关性分析，红光的作用比其他光更显著。
