Ullah Sana, Zuberi Amina, Ullah Imdad, Azzam Mahmoud M
Fisheries and Aquaculture Lab, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Zoology, Division of Science and Technology, University of Education, Lahore 54000, Pakistan.
Toxics. 2024 Sep 12;12(9):664. doi: 10.3390/toxics12090664.
The present study was undertaken to evaluate cypermethrin (CYP)-induced oxidative stress [reactive oxygen species (ROS) and lipid peroxidation (LPO) in gills, muscles, brain, and liver tissues] and DNA damage/genotoxicity (peripheral blood erythrocytes) in a freshwater teleost rohu () and the protective role of vitamin C. The LC of CYP against rohu was found to be 4.5 µg/L in a semi-static culture system through probit analysis. Fingerlings of rohu were distributed into four groups (Group 1st served as a control, fed 35% protein basal diet and was not exposed to CYP; Group 2nd was fed a basal diet and exposed to CYP; Group 3rd and Group 4th were fed diets supplemented with vitamin C at the rate of 100 and 200 mg/kg diet, respectively, and exposed to CYP). Fingerlings were reared on a basal and vitamin C-supplemented diet for 28 days prior to exposure to CYP. The results indicate a time-dependent significant increase in ROS and LPO (indicated by time course increase in TBARS level) as well as DNA damage in terms of number of comets, % DNA in tail, tail moment, tail length, and olive tail moment after exposure to LC of CYP. However, statistically comparable results in both Groups 1st and 4th indicate the protective role of vitamin C. The results reveal the effectiveness of vitamin C as a feed additive for countering pesticides toxicity in . The current study indicates CYP as a potential genotoxicant for fish and classifies SCGE as a reliable and sensitive tool for assessing DNA damage.
本研究旨在评估氯氰菊酯（CYP）诱导的氧化应激[鳃、肌肉、脑和肝组织中的活性氧（ROS）和脂质过氧化（LPO）]以及淡水硬骨鱼印度鲤（）外周血红细胞中的DNA损伤/遗传毒性，同时研究维生素C的保护作用。通过概率分析，在半静态培养系统中发现CYP对印度鲤的LC为4.5 µg/L。将印度鲤幼鱼分为四组（第1组作为对照，投喂35%蛋白质的基础饲料，不暴露于CYP；第2组投喂基础饲料并暴露于CYP；第3组和第4组分别投喂添加了100和200 mg/kg维生素C的饲料，并暴露于CYP）。在暴露于CYP之前，幼鱼在基础饲料和添加维生素C的饲料上饲养28天。结果表明，暴露于CYP的LC后，ROS和LPO（以TBARS水平的时间进程增加表示）以及彗星数量、尾部DNA百分比、尾矩、尾长和橄榄尾矩等方面的DNA损伤呈时间依赖性显著增加。然而，第1组和第4组的统计学可比结果表明了维生素C的保护作用。结果揭示了维生素C作为饲料添加剂对抗印度鲤农药毒性的有效性。当前研究表明CYP是鱼类的潜在遗传毒性物质，并将单细胞凝胶电泳（SCGE）归类为评估DNA损伤的可靠且敏感的工具。
