枯草芽孢杆菌 dTDP-l-鼠李糖生物合成途径酶的结构：dTDP-α-d-葡萄糖 4,6-脱水酶，RfbB。
Structure of the Bacillus anthracis dTDP-l-rhamnose biosynthetic pathway enzyme: dTDP-α-d-glucose 4,6-dehydratase, RfbB.
机构信息
Department of Chemistry and Biochemistry, San Francisco State University, USA.
Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, USA; Center for Structural Genomics of Infectious Diseases (CSGID), USA.
出版信息
J Struct Biol. 2018 May;202(2):175-181. doi: 10.1016/j.jsb.2018.01.006. Epub 2018 Jan 10.
Abstract
Many bacteria require l-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-l-rhamnose, which is produced by the dTDP-l-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.
摘要
许多细菌都需要 l-鼠李糖作为细胞壁的关键组成部分。这种糖通过一种被激活的供体 dTDP-l-鼠李糖被转移到细胞壁上，而这种供体是由 dTDP-l-鼠李糖生物合成途径产生的。我们确定了炭疽杆菌中该途径的第二个酶 dTDP-α-d-葡萄糖 4,6-脱水酶（RfbB）的晶体结构。有趣的是，只有在该途径的第三个酶 RfbC 存在的情况下，RfbB 才会结晶；然而，RfbC 并不存在于晶体中。我们的工作代表了单个革兰氏阳性菌中该途径的四个蛋白质的首次完整结构特征描述。
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