Zhdanov Vladimir P
Section of Biological Physics, Department of Physics, Chalmers University of Technology, Göteborg, Sweden; Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk, Russia.
Biosystems. 2018 Mar;165:52-56. doi: 10.1016/j.biosystems.2018.01.003. Epub 2018 Jan 11.
Nanocarrier-mediated mRNA delivery and release into the cells with subsequent translation to protein is of interest in the context of the development of a new generation of drugs. In particular, this protein can play a role of a transcription factor and be used as a tool to regulate temporarily the genetic networks. The corresponding transient kinetics of gene expression are expected to depend on the mechanism and duration of mRNA release. Assuming the release to be rapid on the time scale of other steps, the author shows theoretically the mRNA-related transient features of gene expression occurring in stable, bistable, and oscillatory regimes in a single cell. Qualitatively, the results obtained are found to be fairly similar to those reported earlier for the situation when the release is slow. Thus, the features of the transient kinetics under consideration appear to be less sensitive to the duration of mRNA release compared to what one might expect.
在新一代药物开发的背景下，纳米载体介导的mRNA递送并释放到细胞中，随后翻译为蛋白质备受关注。特别是，这种蛋白质可以发挥转录因子的作用，并用作临时调节基因网络的工具。基因表达相应的瞬态动力学预计将取决于mRNA释放的机制和持续时间。假设释放在其他步骤的时间尺度上很快，作者从理论上展示了在单个细胞的稳定、双稳和振荡状态下发生的与mRNA相关的基因表达瞬态特征。定性地说，发现所获得的结果与早期报道的释放缓慢时的情况相当相似。因此，与预期相比，所考虑的瞬态动力学特征似乎对mRNA释放的持续时间不太敏感。
