Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China.
Int J Mol Sci. 2023 Mar 17;24(6):5737. doi: 10.3390/ijms24065737.
Huanglongbing (HLB) is one of the most severe citrus diseases in the world, causing huge economic losses. However, efficient methods of protecting citrus from HLB have not yet been developed. microRNA (miRNA)-mediated regulation of gene expression is a useful tool to control plant diseases, but the miRNAs involved in regulating resistance to HLB have not yet been identified. In this study, we found that miR171b positively regulated resistance to HLB in citrus. Upon infection with HLB bacteria, the bacteria were detected in the second month in the control plants. However, in the miR171b-overexpressing transgenic citrus plants, the bacteria could not be detected until the 24th month. RNA-seq data indicated that multiple pathways, such as photosynthesis, plant-pathogen interaction, the MAPK signaling pathway, etc., might be involved in improving the resistance to HLB in miR171b-overexpressing plants compared with the control. Finally, we determined that miR171b could target () genes to downregulate their expression, which then led to promoted resistance to HLB stress. Collectively, our results demonstrate that miR171b plays a positive regulatory role in resistance to citrus HLB, and provides a new insight into the role of miRNAs in the adaptation of citrus to HLB stress.
黄龙病（HLB）是世界上最严重的柑橘病害之一，造成了巨大的经济损失。然而，尚未开发出有效保护柑橘免受 HLB 侵害的方法。miRNA（miRNA）介导的基因表达调控是控制植物病害的有用工具，但参与调节对 HLB 抗性的 miRNAs 尚未被鉴定。在这项研究中，我们发现 miR171b 正向调控柑橘对 HLB 的抗性。在感染 HLB 细菌后，对照植株中的细菌在第二个月被检测到。然而，在 miR171b 过表达转基因柑橘植物中，直到第 24 个月才检测到细菌。RNA-seq 数据表明，与对照相比，多个途径，如光合作用、植物-病原体相互作用、MAPK 信号通路等，可能参与提高 miR171b 过表达植株对 HLB 的抗性。最后，我们确定 miR171b 可以靶向（）基因来下调其表达，从而导致对 HLB 胁迫的抗性增强。总之，我们的结果表明 miR171b 在柑橘对 HLB 的抗性中发挥正向调控作用，并为 miRNA 在柑橘适应 HLB 胁迫中的作用提供了新的见解。
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