Trypanosoma cruzi: modulation of parasite-cell interaction by plasma fibronectin.

Treatment of either rat peritoneal macrophages (RPM), cloned 3T3 fibroblasts (3T3FR) or Trypanosoma cruzi trypomastigote culture forms with human plasma fibronectin (huFN) enhanced their association with the untreated counterpart and this related to the concentration of huFN used. When treatment was performed at 4 degrees C, the enhancing effect of huFN on parasite-cell interaction was greater than that observed at 37 degrees C. This observation could be related to the indirect immunofluorescence antibody assay showing that a significant increase of fibronectin staining was observed on the cell and parasite surfaces upon incubation with huFN and that the extent of fibronectin staining was greater at 4 degrees C. Incubation of huFN-treated or nontreated parasites or cells with anti-huFN antibodies exerted an inhibitory effect on the parasite-cell association. The region of fibronectin that interacts with the trypomastigote surface is unknown. Inhibition experiments suggested that the domain of fibronectin which interacts with parasite surface receptors would probably be localized close to the NH2-terminal region of the molecule. Taken together, these results suggest that fibronectin may play a role in the binding of parasites to the vertebrate host cell surface.