Maryanski J L, Moretta A, Jordan B, De Plaen E, Van Pel A, Boon T, Cerottini J C
Eur J Immunol. 1985 Nov;15(11):1111-7. doi: 10.1002/eji.1830151109.
Cloned genes for human major histocompatibility complex (MHC) class I antigens were introduced by DNA-mediated gene transfer into a high-efficiency transfection recipient (HTR) cell line previously derived from mouse mastocytoma P815. Cell surface expression of HLA-A3, AW24 and CW3 gene products on P815 transfectants was demonstrated by radioimmune assay and by flow cytometry. The human MHC class I gene products were apparently expressed on P815 transfectants in a form recognized by human cytolytic T lymphocytes (CTL). Human CTL generated in unidirectional mixed lymphocyte culture against AW24+ donor lymphocytes clearly lysed P815-HLA-AW24+ transfectant target cells, but not untransfected P815(HTR) controls. Moreover, P815-HLA transfectants could stimulate in vitro a significant alloreactive human CTL response. Lysis of P815-HLA transfectant target cells by human CTL was inhibited by a monoclonal antibody directed against human MHC class I gene products. These mouse cell transfectants may be useful for the study of human T cell responses.
通过DNA介导的基因转移，将人类主要组织相容性复合体（MHC）I类抗原的克隆基因导入先前从小鼠肥大细胞瘤P815衍生而来的高效转染受体（HTR）细胞系。通过放射免疫测定和流式细胞术证明了P815转染细胞上HLA - A3、AW24和CW3基因产物的细胞表面表达。人类MHC I类基因产物显然以人类细胞毒性T淋巴细胞（CTL）可识别的形式在P815转染细胞上表达。在单向混合淋巴细胞培养中针对AW24 +供体淋巴细胞产生的人类CTL能够明显裂解P815 - HLA - AW24 +转染靶细胞，但不能裂解未转染的P815（HTR）对照细胞。此外，P815 - HLA转染细胞能够在体外刺激显著的同种异体反应性人类CTL反应。针对人类MHC I类基因产物的单克隆抗体可抑制人类CTL对P815 - HLA转染靶细胞的裂解。这些小鼠细胞转染体可能对研究人类T细胞反应有用。
