对引入与原始抗原无关基因中的寡核苷酸编码T细胞表位的识别。
Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen.
作者信息
Chimini G, Pala P, Sire J, Jordan B R, Maryanski J L
机构信息
Centre d'Immunologie INSERM, Centre National de Recherche Scientifique de Marseille-Luminy, Marseille, France.
出版信息
J Exp Med. 1989 Jan 1;169(1):297-302. doi: 10.1084/jem.169.1.297.
Abstract
We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.
摘要
我们之前已经证明，针对HLA-CW3或HLA-A24的H-2Kd限制性CTL能够识别与HLA分子第170 - 182位残基相对应的合成肽。将编码CW3或A24第170 - 182位区域的合成寡核苷酸插入流感核蛋白（NP）基因。我们在此证明，用NP-寡核苷酸重组基因转染的P815（H-2d）细胞被HLA特异性Kd限制性CTL克隆特异性裂解。我们的结果表明，在不同分子背景下T细胞表位的表达必定具有高度的灵活性。
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