Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
Department of Nephrology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
Biomed Res Int. 2017;2017:2520347. doi: 10.1155/2017/2520347. Epub 2017 Nov 29.
To enhance nucleoside production in , the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified. The differential expression analysis showed that , and genes involved in purine nucleotide biosynthesis were significantly upregulated 16.56-fold, 8-fold, and 5.43-fold, respectively. Moreover, , , and genes participating in pyrimidine nucleoside biosynthesis were upregulated 4.53-fold, 10.63-fold, 4.26-fold, and 5.98-fold, respectively. To enhance the nucleoside production, precursors for synthesis of nucleosides were added based on the analysis of biosynthetic pathways. Uridine and cytidine contents, respectively, reached 5.04 mg/g and 3.54 mg/g when adding 2 mg/mL of ribose, resulting in an increase of 28.6% and 296% compared with the control, respectively. Meanwhile, uridine and cytidine contents, respectively, reached 10.83 mg/g 2.12 mg/g when adding 0.3 mg/mL of uracil, leading to an increase of 176.3% and 137.1%, respectively. This report indicated that fermentation regulation was an effective way to enhance the nucleoside production in based on biosynthetic pathway analysis.
为了提高 的核苷产量，构建并验证了嘌呤和嘧啶核苷的生物合成途径。差异表达分析表明，参与嘌呤核苷酸生物合成的 、 和 基因分别显著上调了 16.56 倍、8 倍和 5.43 倍。此外，参与嘧啶核苷生物合成的 、 、 和 基因分别上调了 4.53 倍、10.63 倍、4.26 倍和 5.98 倍。为了提高核苷产量，根据生物合成途径分析添加了合成核苷的前体。当添加 2mg/mL 的核糖时，尿苷和胞苷的含量分别达到 5.04mg/g 和 3.54mg/g，与对照相比分别增加了 28.6%和 296%。同时，当添加 0.3mg/mL 的尿嘧啶时，尿苷和胞苷的含量分别达到 10.83mg/g 和 2.12mg/g，与对照相比分别增加了 176.3%和 137.1%。本报告表明，基于生物合成途径分析，发酵调控是提高 的核苷产量的有效途径。
