数分钟内通过基于片段可视化读数对DNA分子进行计数。

Counting DNA molecules with visual segment-based readouts in minutes.

作者信息

Wang Rui, Zhang Fang, Qian Cheng, Wu Cui, Ye Zunzhong, Wang Liu, Qian Wenjuan, Ping Jianfeng, Wu Jian, Ying Yibin

机构信息

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

出版信息

Chem Commun (Camb). 2018 Jan 25;54(9):1105-1108. doi: 10.1039/c7cc09515e.

DOI:10.1039/c7cc09515e

PMID:29333552

Abstract

An ultrafast and extremely simple approach was proposed to count the number of DNA molecules without any microfluidic-based device. By directly counting the number of amplicon clusters in a capillary, the absolute amount of DNA molecules could be easily determined.

摘要

提出了一种超快速且极其简单的方法，无需任何基于微流控的设备即可对DNA分子数量进行计数。通过直接计数毛细管中扩增子簇的数量，可以轻松确定DNA分子的绝对数量。