School of Chemical Sciences, Dublin City University, Glasnevin, Dublin, 9, Ireland.
DSM, P.O. Box 18, 6160, MD, Geleen, the Netherlands.
Macromol Rapid Commun. 2018 Apr;39(7):e1700743. doi: 10.1002/marc.201700743. Epub 2018 Jan 15.
Silicon wafers are decorated with photoamine generator 4,5-dimethoxy-2-nitrobenzyl 3-(triethoxysilyl)propyl carbamate. UV-irradiation in the presence of benzyl-l-glutamate N-carboxyanhydride is carried out, resulting in the release of the surface-bound primary amines, making them viable N-carboxyanhydride (NCA) polymerization initiators. Successful polypeptide grafting is confirmed by water contact angle measurements as well as by ellipsometry, revealing a poly(benzyl-l-glutamate) (PBLG) layer of ≈3 nm. X-ray photoelectron spectroscopy confirms the presence of amide groups in the grafted PBLG while time-of-flight secondary ion mass spectroscopy provides additional evidence for the presence of PBLG on the surface. Evaluation of negative control samples confirms successful UV surface grafting. The approach is thus established as a viable general method for light exposure directable polypeptide functionalization of silicon surfaces.
硅片表面修饰有光胺发生器 4,5-二甲氧基-2-硝基苄基 3-(三乙氧基硅基)丙基氨基甲酸酯。在苯丙氨酸 N-羧基酐的存在下进行 UV 照射，导致表面结合的伯胺释放，从而成为可行的 N-羧基酐（NCA）聚合引发剂。通过水接触角测量和椭圆光度法成功确认多肽接枝，结果表明接枝的聚（苯丙氨酸）（PBLG）层约为 3nm。X 射线光电子能谱（XPS）证实接枝 PBLG 中存在酰胺基团，而飞行时间二次离子质谱（ToF-SIMS）则为表面存在 PBLG 提供了额外的证据。对阴性对照样品的评估证实了成功的 UV 表面接枝。因此，该方法被确立为一种可行的通用方法，可用于硅表面的光可控制多肽功能化。
