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用于人类骨骼肌纤维中三磷酸腺苷酶组织化学显示的铅法的非特异性

The nonspecificity of the lead method for the histochemical demonstration of adenosine triphosphatases in human skeletal muscle fibres.

作者信息

Erzen I, Sirca A

出版信息

J Anat. 1985 Jan;140 ( Pt 1)(Pt 1):13-23.

PMID:2933378
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165132/
Abstract

The lead method for the histochemical demonstration of presumptive mitochondrial adenosinetriphosphatase was applied to biopsy and autopsy samples of the human vastus lateralis muscle. The effect of p-chloromercuribenzoate and of Triton X-100 was tested microdensitometrically and the activity of 'mitochondrial' ATPase was compared to the activity of enzymes of the oxidative metabolism succinic dehydrogenase and NAD-tetrazolium reductase. It is concluded that the ATPase activity displayed is not mainly mitochondrial. In autopsy material, it seems to be predominantly myofibrillar.

摘要

用于组织化学显示假定的线粒体腺苷三磷酸酶的主要方法应用于人类股外侧肌的活检和尸检样本。用微量密度测定法测试了对氯汞苯甲酸和曲拉通X - 100的作用，并将“线粒体”ATP酶的活性与氧化代谢酶琥珀酸脱氢酶和NAD - 四唑还原酶的活性进行了比较。得出的结论是，所显示的ATP酶活性并非主要是线粒体的。在尸检材料中，它似乎主要是肌原纤维的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e72/1165132/d923bbd5388b/janat00193-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e72/1165132/d923bbd5388b/janat00193-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e72/1165132/d923bbd5388b/janat00193-0023-a.jpg

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本文引用的文献

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The specificity of the histochemical method for adenosine triphosphatase.三磷酸腺苷酶组织化学方法的特异性。
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Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphatase.催化氧化磷酸化的酶的部分解析。I. 可溶性二硝基苯酚刺激的三磷酸腺苷酶的纯化及性质
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The enzymic hydrolysis of adenosine triphosphate by liver mitochondria. 2. Effect of inhibitors and added cofactors.肝脏线粒体对三磷酸腺苷的酶促水解作用。2. 抑制剂和添加的辅因子的影响。
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Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole.使用一种新的对硝基苯基取代的双四氮唑对琥珀酸脱氢酶进行细胞化学显示。
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