NOVIKOFF A B, SHIN W Y, DRUCKER J
J Biophys Biochem Cytol. 1961 Jan;9(1):47-61. doi: 10.1083/jcb.9.1.47.
A comparison is made of the staining results obtained with Nitro-BT and MTT-Co(++) as acceptors when DPNH is the substrate in frozen sections of cold formol-calcium-fixed rat kidney (normal and following ligation of the blood vessels) and human liver containing lipofuscin granules. The kidney results are evaluated in terms of mitochondrial morphology seen after classical mitochondrial stains and in electron micrographs. It is concluded that, except for formazan deposition on lipid-aqueous interfaces, Nitro-BT staining indicates the intracellular localization of oxidative enzymes, at least at the level of light microscopy. In contrast, the use of MTT-Co(++) is not reliable for such intracellular localizations. The deposition of the formazan of MTT-Co(++) is determined in large part by physicochemical factors other than enzyme localization. Despite marked abnormalities of the mitochondria in cells of the ligated kidney, MTT-Co(++) formazan is generally deposited in the same dotlike fashion as in cells of normal kidney.
当以二磷酸硫胺素（DPNH）为底物时，比较了在冷甲醛-钙固定的大鼠肾脏（正常及血管结扎后）和含有脂褐素颗粒的人肝脏冰冻切片中，用硝基蓝四氮唑（Nitro-BT）和MTT-钴（++）作为受体所获得的染色结果。根据经典线粒体染色后及电子显微镜下所见的线粒体形态对肾脏结果进行评估。得出的结论是，除了甲臜沉积在脂质-水界面外，Nitro-BT染色至少在光学显微镜水平上表明了氧化酶的细胞内定位。相比之下，MTT-钴（++）用于此类细胞内定位并不可靠。MTT-钴（++）甲臜的沉积在很大程度上由酶定位以外的物理化学因素决定。尽管结扎肾脏细胞中的线粒体有明显异常，但MTT-钴（++）甲臜通常以与正常肾脏细胞相同的点状方式沉积。
