Junka Adam Feliks, Janczura Adriana, Smutnicka Danuta, Mączyńska Beata, Anna Secewicz, Nowicka Joanna, Bartoszewicz Marzenna, Gościniak Grażyna
Department of Microbiology, Medical University, Wrocław, Poland.
Pol J Microbiol. 2012 Sep 28;61(3):191-197.
Roche's xCelligence impedance-measuring instrument is one of a few commercially available systems of such type. According to the best knowledge of authors, instrument was tested so far only for eukaryotic cell research. The aim of this work was to estimate xCELLigence suitability for the microbiological tests, including (i) measurement of morphological changes in eukaryotic cells as a result of bacterial toxin activity, (ii) measurement of bacterial biofilm formation and (iii) impact of antiseptics on the biofilm structure. To test the infuence of bacterial LT enterotoxin on eukaryotic cell lines, Chinese Hamster Ovary (CHO) cell line and reference strain Escherichia coli ATTC 35401 were used. To investigate Roche's instrument ability to measure biofilm formation and impact of antiseptics on its development, Staphylococcus aureus ATTC6538 reference strain was used. The data generated during the experiments indicate excellent ability of xCelligence instrument to detect cytopathic effect caused by bacterial LT endotoxin and to detect staphylococcal biofilm formation. However, interpretation of the results obtained during real-time measurement of antiseptic's bactericidal activity against staphylococcal biofilm, caused many difficulties. xCelligence instrument can be used for real-time monitoring of morphological changes in CHO cells treated with bacterial LT enterotoxin and for real-time measurement of staphylococcal biofilm formation in vitro. Further investigation is necessary to confirm suitability of system to analyze antiseptic's antimicrobial activity against biofilm in vitro.
罗氏的 xCelligence 阻抗测量仪是少数几种此类市售系统之一。据作者所知，该仪器迄今为止仅用于真核细胞研究。这项工作的目的是评估 xCELLigence 在微生物测试中的适用性，包括：（i）测量由于细菌毒素活性导致的真核细胞形态变化；（ii）测量细菌生物膜的形成；（iii）防腐剂对生物膜结构的影响。为了测试细菌 LT 肠毒素对真核细胞系的影响，使用了中国仓鼠卵巢（CHO）细胞系和参考菌株大肠杆菌 ATTC 35401。为了研究罗氏仪器测量生物膜形成以及防腐剂对其生长影响的能力，使用了金黄色葡萄球菌 ATTC6538 参考菌株。实验期间生成的数据表明，xCelligence 仪器具有出色的能力来检测由细菌 LT 内毒素引起的细胞病变效应以及检测葡萄球菌生物膜的形成。然而，在实时测量防腐剂对葡萄球菌生物膜的杀菌活性时，对所得结果的解读存在许多困难。xCelligence 仪器可用于实时监测经细菌 LT 肠毒素处理的 CHO 细胞的形态变化以及体外实时测量葡萄球菌生物膜的形成。需要进一步研究以确认该系统在体外分析防腐剂对生物膜抗菌活性的适用性。
