SpyCatcher-SpyTag介导的量子点对子代杆状病毒进行原位标记以追踪活细胞中的病毒感染
SpyCatcher-SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells.
作者信息
Ke Xianliang, Zhang Yuan, Zheng Fangliang, Liu Yan, Zheng Zhenhua, Xu Yi, Wang Hanzhong
机构信息
Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China.
出版信息
Chem Commun (Camb). 2018 Jan 31;54(10):1189-1192. doi: 10.1039/c7cc08880a.
A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a SpyTag into the viral glycoprotein, followed by specific conjugation with the SpyCatcher protein on modified quantum dots (QDs) through an isopeptide bond. The labelling method is convenient and efficient and shows little attenuation of viral infectivity. Therefore, it is a biologically compatible technique for tracking viral infection.
提出了一种非侵入性标记策略，通过将SpyTag基因插入病毒糖蛋白来标记杆状病毒，然后通过异肽键与修饰量子点（QD）上的SpyCatcher蛋白进行特异性缀合。该标记方法方便高效，且病毒感染力几乎没有衰减。因此，它是一种用于追踪病毒感染的生物相容性技术。
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