基于 His 标签的病毒后代原位标记用于活细胞中单病毒的实时跟踪。

His-tag based in situ labelling of progeny viruses for real-time single virus tracking in living cells.

机构信息

Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China.

Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.

出版信息

Nanoscale. 2016 Nov 10;8(44):18635-18639. doi: 10.1039/c6nr05806j.

DOI:10.1039/c6nr05806j

PMID:27805216

Abstract

Tracking virus infection events in live cells is useful for understanding the mechanism of virus infection, and fluorescent labelling is a critical step. Herein a noninvasive strategy for labelling viruses with His-tags was developed by in situ modifying the cell surface proteins with polypeptides containing His-tags during progeny virus assembly. The His-tagged viruses were further conjugated with Ni-nitrilotriacetate complex modified quantum dots, and retained their infectivity for real-time single virus tracking in living cells.

摘要

追踪活细胞中的病毒感染事件有助于了解病毒感染的机制，而荧光标记是关键步骤。在此，通过在子代病毒组装过程中在细胞表面蛋白原位修饰含 His 标签的多肽，开发了一种非侵入性的用 His 标签标记病毒的策略。随后，将带 His 标签的病毒与 Ni- 次氮基三乙酸复合修饰的量子点进行偶联，并且在活细胞中进行实时单病毒追踪时，保持了其感染性。