State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shenyang, 110016, China.
Nanoscale. 2018 Feb 1;10(5):2285-2290. doi: 10.1039/c7nr07264c.
The optogenetic technique, which allows the manipulation of cellular activity patterns in space and time by light, has transformed the field of neuroscience. However, acquiring multidimensional optogenetic information remains challenging despite the fact that several cellular information detection methods have been proposed. Herein, we present a new method to acquire label-free multidimensional information from optogenetically engineered cells using a graphene transistor. Using a graphene film to form a strong densely packed layer with cells, the cellular action potentials were characterized as light-activated transistor conductance signals, which quantified the multidimensional optogenetic information. Based on this approach, some important cellular optogenetic information, including electrophysiological state, cell concentration, expression levels of opsin and response to variable light intensity, were also precisely detected. Furthermore, the graphene transistor was also used to distinguish cells expressing different channelrhodopsin-2 variants. Our study offers a general detection method of multidimensional optogenetic information for extending the applications of the optogenetic technique and provides a novel sensor for the development of future biological prosthetic devices.
光遗传学技术通过光来操纵细胞活动的时空模式，已经改变了神经科学领域。然而，尽管已经提出了几种细胞信息检测方法，获取多维光遗传学信息仍然具有挑战性。在这里，我们提出了一种新的方法，使用石墨烯晶体管从光遗传工程细胞中获取无标记的多维信息。通过使用石墨烯膜与细胞形成强的致密层，细胞动作电位被表征为光激活晶体管电导信号，从而量化了多维光遗传学信息。基于这种方法，还精确地检测了一些重要的细胞光遗传学信息，包括电生理状态、细胞浓度、视蛋白表达水平以及对可变光强度的反应。此外，石墨烯晶体管还可用于区分表达不同通道蛋白变体的细胞。我们的研究为扩展光遗传学技术的应用提供了一种通用的多维光遗传学信息检测方法，并为未来生物假体设备的发展提供了一种新型传感器。
