环状 RNA 在子宫内膜癌患者血清来源的细胞外囊泡中的表达。
Circular RNA expression in extracellular vesicles isolated from serum of patients with endometrial cancer.
机构信息
Department of Radiation Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, PR China.
Department of Gynecology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, PR China.
出版信息
Epigenomics. 2018 Feb;10(2):187-197. doi: 10.2217/epi-2017-0109. Epub 2018 Jan 15.
AIM
We aimed to explore the roles of circular RNAs (circRNAs) in extracellular vesicles (EVs) isolated from serum of patients with endometrial cancer.
MATERIALS & METHODS: EVs were isolated from serum samples of three patients with stage III adenocarcinoma aged 50-60 years and three matched healthy controls. RNA was extracted from the EVs and analyzed using RNA-seq technique.
RESULTS
We got 209 upregulated circRNAs and 66 downregulated circRNAs. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that the differentially expressed circRNAs were enriched in five pathways. The expression level of hsa_circ_0109046 and hsa_circ_0002577 was confirmed using real-time quantitative PCR.
CONCLUSION
We identified 275 differentially expressed circRNAs and the expression level of two circRNAs was confirmed using real-time quantitative PCR.
目的
本研究旨在探讨来源于子宫内膜癌患者血清外泌体（EVs）中的环状 RNA（circRNAs）的作用。
材料与方法
从 3 名年龄在 50-60 岁之间的 III 期腺癌患者和 3 名匹配的健康对照者的血清样本中分离 EVs。从 EVs 中提取 RNA，并采用 RNA-seq 技术进行分析。
结果
我们共得到 209 个上调的 circRNAs 和 66 个下调的 circRNAs。京都基因与基因组百科全书通路分析表明，差异表达的 circRNAs 富集在五个通路中。采用实时定量 PCR 验证了 hsa_circ_0109046 和 hsa_circ_0002577 的表达水平。
结论
本研究共鉴定了 275 个差异表达的 circRNAs，并采用实时定量 PCR 验证了两个 circRNAs 的表达水平。
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