Fang Song, Tao Yi, Zhang Yizhi, Kong Fanyu, Wang Yunbai
Tobacco Research Institute, Chinese Academy of Agricultural Sciences & Laboratory of Risk Assessment for Tobacco Products (Qingdao), Ministry of Agriculture, Qingdao, Shandong, China.
Chirality. 2018 Apr;30(4):469-474. doi: 10.1002/chir.22810. Epub 2018 Jan 15.
The objective of this experiment was to study the effects of metalaxyl enantiomers on the activity of roots and antioxidative enzymes in tobacco seedlings. Water culture experiment was conducted to analyze the effects of different concentrations of metalaxyl enantiomers (30 and 10 mg L ) on root activity and leaf superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities and malondialdehyde (MDA) content of tobacco seedlings. The results showed that metalaxyl significantly inhibited root activity and significantly improved leaf SOD, POD, and CAT activities and MDA content. A better physiological response in tobacco seedlings was observed at 30 mg L than at 10 mg L metalaxyl. The stereoselectivity for different enantiomers had no obvious effect on root activity and the leaf POD activity, but it affected significantly the SOD and CAT activities and MDA content. The SOD activity was promoted more by R-enantiomer than by S-enantiomer at 30 mg L metalaxyl, and the same effect was observed on CAT activity from the beginning to the end of the stress period. The MDA content under the stress by R-enantiomer was higher than that under the stress by S-enantiomer at 10 mg L metalaxyl.
本实验的目的是研究甲霜灵对映体对烟草幼苗根系活性和抗氧化酶的影响。采用水培实验分析不同浓度甲霜灵对映体（30和10 mg L）对烟草幼苗根系活性、叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性及丙二醛（MDA）含量的影响。结果表明，甲霜灵显著抑制根系活性，显著提高叶片SOD、POD和CAT活性及MDA含量。在30 mg L甲霜灵处理下，烟草幼苗的生理响应优于10 mg L甲霜灵处理。不同对映体的立体选择性对根系活性和叶片POD活性无明显影响，但对SOD和CAT活性及MDA含量有显著影响。在30 mg L甲霜灵处理下，R-对映体对SOD活性的促进作用大于S-对映体，在胁迫期全过程中对CAT活性的影响也相同。在10 mg L甲霜灵处理下，R-对映体胁迫下的MDA含量高于S-对映体胁迫下的MDA含量。
