College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China.
Int J Environ Res Public Health. 2021 Nov 16;18(22):12046. doi: 10.3390/ijerph182212046.
Cadmium (Cd) pollution is a widespread environmental problem. In this study, we explored the transcriptome and biochemical responses of goldenrain tree ( Laxm.) leaves and roots to Cd stress. Leaf and root growth decreased substantially under Cd stress (50 mg/L CdCl), but leaf and root antioxidant mechanisms were significantly activated. In RNA-seq analysis, roots treated with 25 mg/L CdCl featured enriched GO terms in cellular components related to intracellular ribonucleoprotein complex, ribonucleoprotein complex, and macromolecular complex. In leaves under Cd stress, most differentially expressed genes were enriched in the cellular component terms intrinsic component of membrane and membrane part. Weighted gene co-expression network analysis and analysis of module-trait relations revealed candidate genes associated with superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities and malondialdehyde (MDA). Ten transcription factors responded to Cd stress expression, including those in C2H2, MYB, WRKY, and bZIP families. Transcriptomic analysis of goldenrain tree revealed that Cd stress rapidly induced the intracellular ribonucleoprotein complex in the roots and the intrinsic component of membrane in the leaves. The results also indicate directions for further analyses of molecular mechanisms of Cd tolerance and accumulation in goldenrain tree.
镉（Cd）污染是一个广泛存在的环境问题。本研究探讨了金叶树（Laxm.）叶片和根系对镉胁迫的转录组和生化反应。叶片和根系生长在 Cd 胁迫（50mg/L CdCl）下显著下降，但叶片和根系抗氧化机制显著激活。在 RNA-seq 分析中，用 25mg/L CdCl 处理的根富含与细胞内核糖核蛋白复合物、核糖核蛋白复合物和大分子复合物有关的细胞成分的 GO 术语。在 Cd 胁迫下的叶片中，大多数差异表达基因富集在细胞膜和膜部分的细胞成分术语中。加权基因共表达网络分析和模块特征关系分析揭示了与超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性以及丙二醛（MDA）相关的候选基因。十个转录因子对 Cd 胁迫表达有反应，包括 C2H2、MYB、WRKY 和 bZIP 家族的转录因子。金叶树的转录组分析表明，Cd 胁迫迅速诱导根内的核糖核蛋白复合物和叶内的膜固有成分。研究结果还为进一步分析金叶树 Cd 耐受和积累的分子机制指明了方向。
