Noma T, Dorf M E
J Immunol. 1985 Dec;135(6):3655-60.
The ability of antigen-coupled splenic adherent cells to induce suppressor T cells (Ts) is dependent on the presence of I-J determinants on antigen-presenting cells. After 4 days of in vitro culture, antigen-coupled adherent cells lose the capacity to induce Ts. Supernatants from Con A-stimulated lymphocyte cultures and purified interferon-gamma can sustain accessory function for the induction of Ts. Furthermore, after in vitro culture of splenic adherent cells, there is an apparent correlation between the loss of I-A determinants and the decrease in I-J-restricted Ts induction. Stimulation of Ia expression with interferon-gamma results in a simultaneous increase in the ability to induce Ts. Finally, elimination of I-A-bearing splenic adherent cells with antibody + C eliminates I-J-restricted Ts induction. The combined data imply a co-regulation of I-A and I-J on the antigen-presenting cells involved in the induction of both the Ts1 and Ts3 suppressor T cell subsets.
抗原偶联的脾黏附细胞诱导抑制性T细胞（Ts）的能力取决于抗原呈递细胞上I-J决定簇的存在。体外培养4天后，抗原偶联的黏附细胞失去诱导Ts的能力。来自刀豆蛋白A刺激的淋巴细胞培养物的上清液和纯化的干扰素-γ可维持诱导Ts的辅助功能。此外，脾黏附细胞体外培养后，I-A决定簇的丢失与I-J限制的Ts诱导减少之间存在明显的相关性。用干扰素-γ刺激Ia表达会导致诱导Ts的能力同时增加。最后，用抗体+C消除携带I-A的脾黏附细胞可消除I-J限制的Ts诱导。综合数据表明，在参与诱导Ts1和Ts3抑制性T细胞亚群的抗原呈递细胞上，I-A和I-J存在共同调节。
