Hausman P B, Kawasaki H, O'Hara R M, Minami M, Sherr D H, Dorf M E
J Immunol. 1986 Dec 15;137(12):3717-25.
The role of accessory cell populations in the generation of effector suppressor (Ts3) cells was studied. By using an in vitro culture system, it was previously determined that the induction of NP-specific effector suppressor activity requires T cells, antigen, and an anti-idiotypic B cell population. We now demonstrate that the generation of Ts3 cells in this system also requires accessory cells. The accessory population appears to play a role in the processing and presentation of antigen. These antigen-presenting accessory cells are required early in the induction phase of Ts3 generation. These accessory cells can present NP coupled to immunogenic or non-immunogenic polypeptide carriers, including polymers of L-amino acids. However, NP coupled to polymers of poorly metabolized D-amino acids fail to induce suppressor T cell generation. Furthermore, the data demonstrate that an H-2 homology must exist between the Ts3 precursors and the antigen-presenting cell population if suppressor activity is to be generated. We also characterize the differential genetic restrictions that govern the induction of Ts3 cells that control suppression of either T cell or B cell responses. The data suggest that although I-J region encoded gene products control the induction and effector phases of suppressor cell activity as measured on T cell responses, the suppression of B cell responses appear to be controlled by I-A gene products. Possible cellular mechanisms that might explain these findings are discussed.
研究了辅助细胞群体在效应抑制细胞（Ts3）产生中的作用。通过使用体外培养系统，先前已确定NP特异性效应抑制活性的诱导需要T细胞、抗原和抗独特型B细胞群体。我们现在证明，该系统中Ts3细胞的产生也需要辅助细胞。辅助细胞群体似乎在抗原的加工和呈递中发挥作用。这些抗原呈递辅助细胞在Ts3产生的诱导阶段早期是必需的。这些辅助细胞可以呈递与免疫原性或非免疫原性多肽载体偶联的NP，包括L-氨基酸聚合物。然而，与代谢不良的D-氨基酸聚合物偶联的NP不能诱导抑制性T细胞的产生。此外，数据表明，如果要产生抑制活性，Ts3前体与抗原呈递细胞群体之间必须存在H-2同源性。我们还描述了控制T细胞或B细胞反应抑制的Ts3细胞诱导的不同遗传限制。数据表明，虽然I-J区域编码的基因产物控制着对T细胞反应所测定的抑制细胞活性的诱导和效应阶段，但B细胞反应的抑制似乎由I-A基因产物控制。讨论了可能解释这些发现的细胞机制。
