一种针对φX174单链DNA的单克隆抗体的新型竞争性检测方法。
A new competitive assay for monoclonal antibodies against phi X174 single stranded DNA.
作者信息
Yoshikawa K, Fukada M
出版信息
J Immunol Methods. 1985 Nov 28;84(1-2):45-52. doi: 10.1016/0022-1759(85)90413-2.
A new method using L-lysine-conjugated Sepharose 4B as a matrix for coupling competitors is described for analysis of the specificity of monoclonal antibodies against single stranded DNA. In this assay, competitor-coupled Sepharose 4B is first incubated with an antibody, and then the competitor is removed (together with the antibody that is bound to it) by low-speed centrifugation of the reaction mixture. The supernatant containing only unreacted antibody is then analysed by an enzyme-linked immunosorbent assay. The method is simple and does not use radioactive materials. Moreover it is not subject to the data fluctuation that is caused by the binding of competitors to microplates in a commonly used enzyme-linked immunosorbent assay employing high concentrations of competitors.
本文描述了一种新方法，该方法使用L-赖氨酸偶联的琼脂糖4B作为偶联竞争物的基质，用于分析抗单链DNA单克隆抗体的特异性。在该测定中，首先将偶联竞争物的琼脂糖4B与抗体孵育，然后通过对反应混合物进行低速离心去除竞争物（以及与之结合的抗体）。然后通过酶联免疫吸附测定法分析仅含有未反应抗体的上清液。该方法简单，不使用放射性物质。此外，它不会受到在常用的使用高浓度竞争物的酶联免疫吸附测定中竞争物与微孔板结合所引起的数据波动的影响。
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