Lafleur M V, Woldhuis J, Loman H
Nucleic Acids Res. 1981 Dec 11;9(23):6591-9. doi: 10.1093/nar/9.23.6591.
With biologically active DNA of the bacteriophage phi X174, both single and double-stranded, some physico-chemical and biological parameters of the depurination reaction are studied. It is shown that in single-stranded DNA each apurinic site is lethal, while in double-stranded RFI-DNA only about 5% of these sites are lethal. Furthermore it is concluded that the apurinic sites are formed at different rates in single- and double-stranded DNA and also the conversion into breaks of the apurinic sites is different for both forms of DNA.
利用噬菌体φX174的生物活性DNA（单链和双链），研究了脱嘌呤反应的一些物理化学和生物学参数。结果表明，在单链DNA中，每个脱嘌呤位点都是致死性的，而在双链RFI-DNA中，只有约5%的这些位点是致死性的。此外，得出的结论是，脱嘌呤位点在单链和双链DNA中以不同的速率形成，并且两种形式的DNA中脱嘌呤位点向断裂的转化也不同。
