Department of Chemistry and ‡MIT BioMicroCenter, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Massachusetts 02139, United States.
J Am Chem Soc. 2018 Feb 21;140(7):2413-2416. doi: 10.1021/jacs.7b12897. Epub 2018 Feb 12.
We describe the preparation, evaluation, and application of an S100A12 protein-conjugated solid support, hereafter the "A12-resin", that can remove 99% of Zn(II) from complex biological solutions without significantly perturbing the concentrations of other metal ions. The A12-resin can be applied to selectively deplete Zn(II) from diverse tissue culture media and from other biological fluids, including human serum. To further demonstrate the utility of this approach, we investigated metabolic, transcriptomic, and metallomic responses of HEK293 cells cultured in medium depleted of Zn(II) using S100A12. The resulting data provide insight into how cells respond to acute Zn(II) deficiency. We expect that the A12-resin will facilitate interrogation of disrupted Zn(II) homeostasis in biological settings, uncovering novel roles for Zn(II) in biology.
我们描述了一种 S100A12 蛋白偶联固相的制备、评估和应用，简称“ A12 树脂”，它可以在不显著改变其他金属离子浓度的情况下，从复杂的生物溶液中去除 99%的 Zn(II)。A12 树脂可用于选择性地从各种组织培养基和其他生物液（包括人血清）中去除 Zn(II)。为了进一步证明这种方法的实用性，我们使用 S100A12 研究了在 Zn(II)耗尽的培养基中培养的 HEK293 细胞的代谢组学、转录组学和金属组学反应。得到的数据提供了关于细胞如何响应急性 Zn(II)缺乏的见解。我们预计 A12 树脂将有助于在生物环境中研究破坏的 Zn(II)动态平衡，揭示 Zn(II)在生物学中的新作用。
