Jang Su, Lee Yunjoo, Lee Gileung, Seo Jeonghwan, Lee Dongryung, Yu Yoye, Chin Joong Hyoun, Koh Hee-Jong
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 08826, South Korea.
Graduate School of Integrated Bioindustry, Sejong University, 209, Neungdong-ro, Gwangjin-gu, Seoul, 05006, South Korea.
BMC Genet. 2018 Jan 15;19(1):5. doi: 10.1186/s12863-017-0591-6.
Balancing panicle-related traits such as panicle length and the numbers of primary and secondary branches per panicle, is key to improving the number of spikelets per panicle in rice. Identifying genetic information contributes to a broader understanding of the roles of gene and provides candidate alleles for use as DNA markers. Discovering relations between panicle-related traits and sequence variants allows opportunity for molecular application in rice breeding to improve the number of spikelets per panicle.
In total, 142 polymorphic sites, which constructed 58 haplotypes, were detected in coding regions of ten panicle development gene and 35 sequence variants in six genes were significantly associated with panicle-related traits. Rice cultivars were clustered according to their sequence variant profiles. One of the four resultant clusters, which contained only indica and tong-il varieties, exhibited the largest average number of favorable alleles and highest average number of spikelets per panicle, suggesting that the favorable allele combination found in this cluster was beneficial in increasing the number of spikelets per panicle.
Favorable alleles identified in this study can be used to develop functional markers for rice breeding programs. Furthermore, stacking several favorable alleles has the potential to substantially improve the number of spikelets per panicle in rice.
平衡穗相关性状，如穗长以及每穗一次枝梗和二次枝梗数量，是提高水稻每穗颖花数的关键。鉴定遗传信息有助于更广泛地理解基因的作用，并提供用作DNA标记的候选等位基因。发现穗相关性状与序列变异之间的关系为水稻育种中的分子应用提供了机会，以提高每穗颖花数。
在10个穗发育基因的编码区共检测到142个多态性位点，构建了58种单倍型，6个基因中的35个序列变异与穗相关性状显著相关。水稻品种根据其序列变异图谱进行聚类。四个聚类结果之一，仅包含籼稻和通系品种，表现出最大的平均有利等位基因数和最高的每穗平均颖花数，表明在该聚类中发现的有利等位基因组合有利于增加每穗颖花数。
本研究中鉴定出的有利等位基因可用于开发水稻育种计划的功能标记。此外，堆叠多个有利等位基因有可能大幅提高水稻每穗颖花数。
