Department of Oral Pathology, Dental Science Research Institute and Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju 61186, Korea.
Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
BMB Rep. 2018 Apr;51(4):188-193. doi: 10.5483/bmbrep.2018.51.4.209.
Caffeoylserotonin (CaS), one derivative of serotonin (5-HT), is a secondary metabolite produced in pepper fruits with strong antioxidant activities. In this study, we investigated the effect of CaS on proliferation and migration of human keratinocyte HaCaT cells compared to that of 5-HT. CaS enhanced keratinocyte proliferation even under serum deficient condition. This effect of CaS was mediated by serotonin 2B receptor (5-HT2BR) related to the cell proliferation effect of 5-HT. We also confirmed that both CaS and 5-HT induced G1 progression via 5-HT2BR/ERK pathway in HaCaT cells. However, Akt pathway was additionally involved in upregulated expression levels of cyclin D1 and cyclin E induced by CaS by activating 5-HT2BR. Moreover, CaS and 5-HT induced cell migration in HaCaT cells via 5-HT2BR. However, 5-HT regulated cell migration only through ERK/AP-1/MMP9 pathway while additional Akt/NF-κB/MMP9 pathway was involved in the cell migration effect of CaS. These results suggest that CaS can enhance keratinocyte proliferation and migration. It might have potential as a reagent beneficial for wound closing and cell regeneration. [BMB Reports 2018; 51(4): 188-193].
咖啡酰血清素（CaS）是 5-羟色胺（5-HT）的一种衍生物，是在辣椒果实中产生的具有强抗氧化活性的次生代谢物。在这项研究中，我们研究了 CaS 对人角质形成细胞 HaCaT 细胞增殖和迁移的影响，与 5-HT 的作用进行了比较。CaS 甚至在血清缺乏的情况下增强了角质形成细胞的增殖。这种 CaS 的作用是通过与 5-HT 的细胞增殖作用相关的 5-羟色胺 2B 受体（5-HT2BR）介导的。我们还证实，CaS 和 5-HT 均通过 5-HT2BR/ERK 通路诱导 HaCaT 细胞中的 G1 期进展。然而，Akt 通路还参与了 CaS 诱导的 cyclin D1 和 cyclin E 上调表达水平，通过激活 5-HT2BR。此外，CaS 和 5-HT 通过 5-HT2BR 诱导 HaCaT 细胞迁移。然而，5-HT 仅通过 ERK/AP-1/MMP9 通路调节细胞迁移，而 Akt/NF-κB/MMP9 通路参与了 CaS 的细胞迁移作用。这些结果表明，CaS 可以增强角质形成细胞的增殖和迁移。它可能具有作为一种有益的试剂的潜力，有利于伤口闭合和细胞再生。[BMB 报告 2018;51(4):188-193]。
