Higa T, Kitamura K, Miyata A, Kangawa K, Matsuo H, Tanaka K
Jpn Circ J. 1985 Sep;49(9):973-9. doi: 10.1253/jcj.49.973.
The cardiac content and plasma concentration of atrial natriuretic polypeptide (ANP) in hypertensive rats were measured by using radioimmunoassay for ANP. The animals used in this study were young (5-week-old) and adult (12- to 14-week-old) spontaneously hypertensive rats (SHR), stroke-prone SHR (SHR-SP) and normotensive control Wistar-Kyoto rats (WKY). Most immunoreactive ANP in the cardiac extract of the hypertensive rats (both SHR and SHR-SP) was found to be predominantly gamma-form as well as that of WKY. Cardiac ANP content in adult SHR and SHR-SP was significantly lower than in adult WKY (p less than 0.01), although there were no differences among three groups in young rats. Similar tendencies were also observed for plasma ANP concentration. The level of plasma in adult hypertensive rats was significantly lower or tended to be lower than in adult normotensive rats. Furthermore, a high negative correlation was present between blood pressure and cardiac ANP content (r = 0.748, p less than 0.01) and between blood pressure and plasma ANP concentration (r = 0.608, p less than 0.01) in all adult rats but there were no such relationships in young rats. The present study suggests that atrial natriuretic polypeptide may contribute to the pathogenesis or maintenance of high blood pressure in the genetic hypertensive rats.
采用心房利钠多肽（ANP）放射免疫分析法，测定了高血压大鼠心脏中ANP含量及血浆浓度。本研究中使用的动物为年轻（5周龄）和成年（12至14周龄）的自发性高血压大鼠（SHR）、易卒中型SHR（SHR-SP）以及血压正常的对照Wistar-Kyoto大鼠（WKY）。结果发现，高血压大鼠（SHR和SHR-SP）心脏提取物中的大多数免疫反应性ANP与WKY大鼠一样，主要为γ型。成年SHR和SHR-SP的心脏ANP含量显著低于成年WKY（p<0.01），不过幼鼠三组之间并无差异。血浆ANP浓度也观察到类似趋势。成年高血压大鼠的血浆水平显著低于或趋于低于成年血压正常大鼠。此外，在所有成年大鼠中，血压与心脏ANP含量之间存在高度负相关（r = 0.748，p<0.01），血压与血浆ANP浓度之间也存在高度负相关（r = 0.608，p<0.01），但幼鼠中不存在这种关系。本研究表明，心房利钠多肽可能在遗传性高血压大鼠高血压的发病机制或维持过程中发挥作用。
