精氨酸加压素-水通道蛋白-2 通路介导电针对 AVP 诱导的内淋巴积水豚鼠模型的脱水作用。
Arginine Vasopressin-Aquaporin-2 Pathway-Mediated Dehydration Effects of Electroacupuncture in Guinea Pig Model of AVP-Induced Eendolymphatic Hydrops.
机构信息
Department of Acupuncture and Moxibustion, the Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Department of Acupuncture and Rehabilitation, Guangxing Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, 310007, China.
出版信息
Chin J Integr Med. 2019 Oct;25(10):763-769. doi: 10.1007/s11655-017-2411-2. Epub 2018 Jan 15.
OBJECTIVE
To investigate the effects of electroacupuncture (EA) on endolymphatic hydrops (EH) and the regulation of arginine vasopressin (AVP)-aquaporin-2 (AQP2) pathway in guinea pigs.
METHODS
EH was induced in male guinea pigs by an intraperitoneal injection of AVP. For the treatment, EA was delivered to Baihui (GV 20) and Tinggong (SI 19) acupoints, once per day for 10 consecutive days. In histomorphological studies, cochlear hydrops degree was evaluated by hematoxylin-eosin (HE) staining, and then the ratio of scala media (SM) area to SM + scala vestibuli (SV) area (R value) was calculated. In mechanical studies, a comparison of plasma AVP (p-AVP) concentrations, cyclic adenosine monophosphate (cAMP) levels, vasopressin type 2 receptor (V2R) and AQP2 mRNA expressions in the cochlea were compared among groups.
RESULTS
EA significantly reduced cochlear hydrops in guinea pigs (P=0.001). EA significantly attenuated the AVPinduced up-regulation of p-AVP concentrations (P=0.006), cochlear cAMP levels (P=0.003) and AQP2 mRNA expression (P=0.016), and up-regulated the expression of V2R mRNA (P=0.004) in the cochlea.
CONCLUSIONS
The dehydrating effect of EA might be associated with its inhibition of AVP-AQP2 pathway activation.
目的
探讨电针对内淋巴积水（EH）的影响及其对精氨酸加压素（AVP）-水通道蛋白-2（AQP2）通路的调节作用。
方法
通过腹腔注射 AVP 诱导雄性豚鼠 EH。治疗时，每日一次，在百会（GV 20）和听宫（SI 19）穴位给予电针治疗，连续 10 天。在组织形态学研究中，通过苏木精-伊红（HE）染色评估耳蜗积水程度，然后计算中阶（SM）面积与 SM +前庭阶（SV）面积的比值（R 值）。在力学研究中，比较各组豚鼠血浆 AVP（p-AVP）浓度、环磷酸腺苷（cAMP）水平、血管加压素 2 型受体（V2R）和 AQP2 基因在耳蜗中的表达。
结果
电针显著减轻豚鼠耳蜗积水（P=0.001）。电针显著抑制 AVP 诱导的 p-AVP 浓度（P=0.006）、耳蜗 cAMP 水平（P=0.003）和 AQP2 mRNA 表达（P=0.016）的上调，并上调 V2R mRNA 的表达（P=0.004）在耳蜗中。
结论
电针的脱水作用可能与其抑制 AVP-AQP2 通路的激活有关。
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