Fujisawa S, Masuhara E
Shika Rikogaku Zasshi. 1979 Jul;20(51):142-50.
In order to evaluate in vitro biological properties of dental cements sixteen different cements including zinc oxide eugenol (ZOE), EBA, resin, polycarboxylate, glass ionomer cement and calcium hydroxide base material were tested regarding elution and binding by bovine serum albumin (BSA). The persistency of elution over long time after subsequent transfer to fresh water was calculated at 210 nm absorbance with ultra violet spectrometer. Also the eluates obtained were analyzed by high performance liquid chromatograpy. The continuous leaching of eugenol from ZOE cement was observed. Binding of eluates from cement to BSA was evaluated spectrophotometrically by utilizing the competition with 2-(4' hydroxyphenylazo) benzoic acid (HABA). EBA cement showed the highest binding ability by BSA because of the leaching o-ethoxybenzoic acid from this cement. The relevance of protein and pulp toxicity is discussed.
为了评估牙科粘固剂的体外生物学特性，对16种不同的粘固剂进行了测试，包括氧化锌丁香酚（ZOE）、EBA、树脂、聚羧酸锌、玻璃离子粘固剂和氢氧化钙基材料，检测其对牛血清白蛋白（BSA）的洗脱和结合情况。随后转移至淡水中后，使用紫外光谱仪在210nm吸光度下计算长时间洗脱的持续性。此外，对获得的洗脱液进行了高效液相色谱分析。观察到ZOE粘固剂中丁香酚的持续浸出。通过利用与2-（4'-羟基苯偶氮）苯甲酸（HABA）的竞争，用分光光度法评估粘固剂洗脱液与BSA的结合。由于该粘固剂中邻乙氧基苯甲酸的浸出，EBA粘固剂显示出最高的BSA结合能力。讨论了蛋白质和牙髓毒性的相关性。
