牙科黏固剂对变形链球菌和嗜酸乳杆菌抗菌作用的比较评价：一项体外研究

Comparative Evaluation of Antibacterial Effect of Dental Luting Cements on Streptococcus mutans and Lactobacillus acidophilus: An In vitro Study.

作者信息

Feroz Sma, Bhoyar A, Khan S

机构信息

Department of Prosthetic Dental Sciences, College of Dentistry Jazan University, Jazan, Kingdom of Saudi Arabia, Phone: +966562793440, e-mail:

Department of Prosthodontics, Crown & Bridge, People's College of Dental Sciences & Research Centre, Bhopal, Madhya Pradesh, India.

出版信息

J Contemp Dent Pract. 2016 Dec 1;17(12):973-977.

PMID:27965482

Abstract

INTRODUCTION

This study aimed at evaluating and comparing the antibacterial activity of six types of dental luting cements on Streptococcus mutans and Lactobacillus acidophilus using the agar diffusion test (ADT) and the direct-contact test (DCT). The antibacterial activity in ADT was measured based on the diameter of the zone of inhibition formed, whereas in DCT the density of the bacterial suspension was measured. The lower the density of the suspension, the more antibacterial activity the cement possesses.

MATERIALS AND METHODS

Agar diffusion test was carried out on the bacteria. After an incubation period of 24 hours, the plates were checked for the presence of zone of inhibition. In DCT the cement was mixed and applied. Once the cement was set, bacterial suspension and brain-heart infusion medium was poured and incubated for 24 hours. After 24 hours, the plate was placed in the enzyme-linked immunosorbent assay plate reader, which measured the optical density of the fluid. The first set of data was recorded approximately 1 hour after incubation. Overall, three sets of data were recorded. Additional experiments were performed on set test materials that were allowed to age for 24 hours, 1 week, 1, 3, and 6 months.

RESULTS

When using ADT only two cements zinc oxide eugenol (ZOE) and zinc polycarboxylate (ZPC) cement showed antibacterial activity against the test organisms. When using DCT, all cements showed some amount of antibacterial activity. Zinc oxide eugenol and ZPC cement showed highest amount of antibacterial activity against S. mutans and L. acidophilus respectively.

CONCLUSION

Within the limitations of study, ZOE cement and ZPC cement were most effective against the tested microorganisms followed by the newer resin cement. The glass ionomer cement was the weakest of all.

CLINICAL SIGNIFICANCE

Patients with high caries index can be treated more effectively using the abovementioned cements.

摘要

引言

本研究旨在通过琼脂扩散试验（ADT）和直接接触试验（DCT）评估并比较六种牙科粘结水门汀对变形链球菌和嗜酸乳杆菌的抗菌活性。ADT中的抗菌活性通过形成的抑菌圈直径来衡量，而在DCT中则通过测量细菌悬液的密度来衡量。悬液密度越低，水门汀的抗菌活性越强。

材料与方法

对细菌进行琼脂扩散试验。培养24小时后，检查平板上是否存在抑菌圈。在DCT中，将水门汀混合并应用。水门汀凝固后，倒入细菌悬液和脑心浸液培养基并培养24小时。24小时后，将平板放入酶联免疫吸附测定酶标仪中，测量液体的光密度。在培养后约1小时记录第一组数据。总体而言，记录了三组数据。对经过24小时、1周、1、3和6个月老化的试验材料进行了额外实验。