School of Pharmacy, Binzhou Medical University , Yantai 264003, China.
Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai 264003, China.
ACS Sens. 2018 Feb 23;3(2):378-385. doi: 10.1021/acssensors.7b00804. Epub 2018 Jan 26.
We describe a simple and effective strategy to construct a molecular imprinting ratiometric fluorescence sensor (MIR sensor) for the visual detection of bovine hemoglobin (BHb) used as a model protein. The sensor was prepared by simply mixing the solution of green and red CdTe quantum dots (QDs), which were embedded in core-shell structured molecularly imprinted polymers and silica nanoparticles, respectively. The resultant hybrid MIR sensor can selectively bind with BHb and thus quench the fluorescence of the green QDs, while the red QDs wrapped with silica are insensitive to BHb with the fluorescence intensity unchanged. As a result, a continuous obvious fluorescence color change from green to red can be observed by naked eyes, with the detection limit of 9.6 nM. Moreover, the MIR sensor was successfully applied to determine BHb in bovine urine samples with satisfactory recoveries at three spiking levels ranging from 95.7 to 101.5%, indicating great potential application for detecting BHb in real samples. This strategy of using different fluorescence emission materials incorporated to construct a ratiometric fluorescence sensor is reasonable and convenient, which can be extended to the preparation of other ratiometric fluorescence systems for targeted analytes.
我们描述了一种简单有效的策略，用于构建用于视觉检测牛血红蛋白 (BHb) 的分子印迹比率荧光传感器 (MIR 传感器)，BHb 用作模型蛋白。传感器是通过简单地混合绿色和红色 CdTe 量子点 (QDs) 的溶液来制备的，这些 QDs 分别嵌入核壳结构的分子印迹聚合物和硅纳米粒子中。所得的混合 MIR 传感器可以选择性地与 BHb 结合，从而猝灭绿色 QDs 的荧光，而用硅包裹的红色 QDs 对 BHb 不敏感，荧光强度保持不变。因此，可以通过肉眼观察到从绿色到红色的连续明显的荧光颜色变化，检测限为 9.6 nM。此外，该 MIR 传感器成功地应用于牛尿液样品中 BHb 的测定，在三个加标水平（95.7%至 101.5%）的回收率令人满意，表明其在实际样品中检测 BHb 具有很大的应用潜力。这种使用不同荧光发射材料结合构建比率荧光传感器的策略是合理且方便的，可扩展到其他针对目标分析物的比率荧光系统的制备中。
