Cai Lei, Zhang Zhaohui, Xiao Haimei, Chen Shan, Fu Jinli
National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Chemical Engineering, Jishou University Jishou 416000 China
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University Changsha 410082 China.
RSC Adv. 2019 Dec 13;9(71):41383-41391. doi: 10.1039/c9ra08726e.
An eco-friendly fluorescent molecularly imprinted polymer anchored on the surface of graphene quantum dots (GQDs@MIP) was developed with an efficient sol-gel polymerization for highly sensitive and selective determination of -nitroaniline (-NA). The GQDs@MIP was characterized in detail by Fourier-transform infrared, fluorescence spectrometer, scanning electron microscope, transmission electron microscope and ultraviolet spectrophotometer. The results showed that the imprinted layer was successfully grafted on the surface of the GQDs. The fluorescence of the GQDs@MIP is efficiently quenched when -NA recombines with the imprinting sites based on the photo-induced electron transfer fluorescence quenching mechanism. A good linear relationship was obtained between the fluorescence quenching efficiency of the GQDs@MIP and the concentration of -NA in the range of 0-15.0 μM with a correlation coefficient of 0.99. The practicability of the proposed method in real samples was successfully evaluated through monitoring -NA in water and fish samples with satisfactory recovery. The developed method provides a feasible and eco-friendly strategy to fabricate MIPs anchored on GQDs with good fluorescence properties for sensitive detection of organic pollutants in complex samples.
通过高效的溶胶 - 凝胶聚合反应，制备了一种锚定在石墨烯量子点表面的环保型荧光分子印迹聚合物（GQDs@MIP），用于高灵敏度和高选择性地测定对硝基苯胺（p-NA）。利用傅里叶变换红外光谱仪、荧光光谱仪、扫描电子显微镜、透射电子显微镜和紫外可见分光光度计对GQDs@MIP进行了详细表征。结果表明，印迹层成功接枝在GQDs表面。基于光致电子转移荧光猝灭机理，当p-NA与印迹位点结合时，GQDs@MIP的荧光被有效猝灭。在0 - 15.0 μM范围内，GQDs@MIP的荧光猝灭效率与p-NA浓度呈现良好的线性关系，相关系数为0.99。通过监测水和鱼样中的p-NA，成功评估了该方法在实际样品中的实用性，回收率令人满意。所开发的方法为制备具有良好荧光性能的锚定在GQDs上的分子印迹聚合物以灵敏检测复杂样品中的有机污染物提供了一种可行且环保的策略。
