Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
Int J Biol Macromol. 2018 May;111:1238-1244. doi: 10.1016/j.ijbiomac.2018.01.057. Epub 2018 Jan 12.
Marine seaweeds contain a valuable source of functional bioactive polysaccharide and it plays main role for effective anticancer activity. The structural feature of SPs was studied through FT-IR and H NMR spectra analysis. The isolated SPs from A. spicifera contain 63.3% of total sugar, 21.9% of total sulfate and 12.6% of total uranic acid was found. The active F2 fraction molecular weight of SP was found to be 420 kDa. The sugar was composed of galactose (73.5%), xylose (9.2%), mannose (1.9%) and arabinose (10.9%). Further the SP showed DPPH free radical scavenging activity of 55.55% at 150 μg/mL and reducing power activity of 91.3% at 125 μg/mL. In the present study, the purified sulfated polysaccharide (fraction F2) were extracted, purified and characterized for red seaweed and evaluated for their potential anticancer activity of in A549 cell lines under in vitro condition. These polysaccharide fractions exhibited potential apoptotic effects on A549 cell lines.
海洋藻类含有有价值的功能性生物活性多糖资源，它对有效的抗癌活性起着主要作用。通过傅里叶变换红外光谱（FT-IR）和核磁共振（1H NMR）光谱分析研究了 SPs 的结构特征。从角叉菜中分离得到的 SPs 含有 63.3%的总糖、21.9%的总硫酸盐和 12.6%的总尿酸。发现 SP 的活性 F2 馏分分子量为 420 kDa。糖由半乳糖（73.5%）、木糖（9.2%）、甘露糖（1.9%）和阿拉伯糖（10.9%）组成。进一步研究表明，SP 在 150μg/mL 时对 DPPH 自由基的清除活性为 55.55%，在 125μg/mL 时的还原力活性为 91.3%。在本研究中，从红海藻中提取、纯化并表征了纯化的硫酸化多糖（F2 馏分），并在体外条件下评估了其在 A549 细胞系中的潜在抗癌活性。这些多糖片段对 A549 细胞系表现出潜在的凋亡作用。
