School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China.
School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China.
Int J Biol Macromol. 2018 Dec;120(Pt A):1087-1092. doi: 10.1016/j.ijbiomac.2018.08.160. Epub 2018 Aug 28.
A novel water-soluble polysaccharide coded APP3a was isolated and purified from Auricularia polytricha by DEAE-52 cellulose and Sephadex G-200 column chromatography. Chemical characterization of APP3a was investigated based on analysis of high performance liquid chromatography (HPLC), gas chromatography (GC), fourier transform infrared spectroscopy (FT-IR) and thermal gravimetric analysis (TGA). Antioxidant activities of APP3a were investigated on the basis of superoxide anion,1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging activity and reducing power. The results showed that APP3a consisted of arabinose, mannose, glucose and galactose in a molar ratio of 1:1.33:1.06:1.23 with an average molecular weight of 21,242 Da. Thermal gravimetric analysis showed that APP3a was relatively stable below 250 °C. APP3a showed the stronger reducing power and stronger scavenging activity against hydroxyl radicals, superoxide radicals and DPPH radicals in a concentration-dependent manner. These findings suggested that the polysaccharide APP3a could be served as a novel antioxidant in functional food.
从黑木耳中分离纯化出一种新型水溶性多糖 APP3a，采用 DEAE-52 纤维素和 Sephadex G-200 柱层析进行分离纯化。根据高效液相色谱（HPLC）、气相色谱（GC）、傅里叶变换红外光谱（FT-IR）和热重分析（TGA）分析，对 APP3a 的化学性质进行了研究。根据超氧阴离子、1,1-二苯基-2-苦基肼（DPPH）、羟基自由基清除活性和还原能力，研究了 APP3a 的抗氧化活性。结果表明，APP3a 由阿拉伯糖、甘露糖、葡萄糖和半乳糖组成，摩尔比为 1:1.33:1.06:1.23，平均分子量为 21,242 Da。热重分析表明，APP3a 在 250°C 以下相对稳定。APP3a 具有较强的还原能力，对羟基自由基、超氧阴离子自由基和 DPPH 自由基的清除活性呈浓度依赖性。这些发现表明，多糖 APP3a 可以作为功能性食品中的一种新型抗氧化剂。
